A mongreld og 2yearsa nd 4months oldw as submittewdi thm ajors ignos fp ersistebnlte edingfrom the nostril and slight deformity of the nasal cavity. A diagnosis of nasal and paranasal sarcoma was made in it from the clinical signs, roentgenography, cytological observation of a nasal excretion, and histological examination of bioptic materials from the mass in the nasal cavity. This tumor was invading from the right nasal cavity into the nasal hone, turbinates, nasal septum, maxillary and frontal sinuses. No complete extirpation of the tumor and involved tissues could be made by curettage, which could not be performedin the deep and narrow parts of the maxillary and frontal sinuses. Therefore, x-ray radiation was applied to the remainder of the tumor cells. About one year and 3months after the treatment, roentgenography and cytological examination of nasal swabs revealed no recurrence of the condition.
A progesterone releasing intravaginal device with an estradiol benzoate capsule (PRID) wasapplied to Holstein cows. 1) In 4 cows in postparturient anestrus PRID was inserted into the vagina for 12days and 25mg of prostaglandin F2α(PGF2α) was injected intramuscularly 24hours before the removal of PRID. All the cows, exceptone, showed standing estrus within 48hours after this removal. The estrous cows were inseminated artificiallyonce58hours after the removal and became conceived, except one. 2) In 8 cows showing an abnormal estrous cycle, PRID was inserted for 12days and superovulation treated with PMSG or FSH and PGF2α48hours before the removal of PRID. The mean numbers of ova collected and normal embryos per donor were 4.4 and 2.9, respectively, and 66% of ova collected from the donors with superovulation was judged as normal. 3) In 5 donor cows 1 to 4days after collection following the usual superovulation scheme (first) PRID was inserted for 14days. Superovulation was treated again with PMSG or FSH in combination with PGF2αtreatment 2days before the removal of PRID (second). The proportion of normal embryos was 65.5 and 40.0% in the first and second treatment, respectively. In conclusion, the PRID treatment was effective for the recurrence of estrus in postpartum anestrus and for superovulation in cows showing an abnormal estrouscycle.
Total whey protein was considered to be useful for the diagnosis of the type of Mastitis. Two simple methods were devised to determine total whey protein. Bromophenol blue (BPB) tube method (temporary name): Mix 0.5ml of 0.2M acetic acid buffer solution, pH4.0, and 0.5ml of milk Centrifuge at 2, 000rpm for 5minutes Isolate whey. Dilute whey 10-20times with acetic acid buffer solution. Mix 1ml of diluted whey and 4.0ml of BPB solution (dissolve 5mg of BPB with 100ml of 0.2M citrate-HCI buffer solutionpH3.0). Determine with spectrophotometer at wavelength of 610μm. BPB filter paper method (temporary name): Mix 0.2M acetic acid buffer solution, pH4.0, and an equal volume ofmilk. Add 5μl of mixed solution to BPB filter paper (dissolve 100mg of BPB with 100ml of 1.0% citrate solution. Dip a piece of filter paper in the solution and dry). Compare the color and yellow ring size with the standard (made with bovine serum albumin).
In pigs which showed rapid rigor mortis in usual or experimental slaughter, quite remarkable changes were found (enzymes: CPK, GOT, GPT, α-HBD, LDH and their isozymes, lactic acid, lipids: TG, NEFA, and minerals: Pi, Ca) in blood and glycogen and lactic acid, decreasing rate of muscle glycogen, ATP, catabolizing pattern of ATP, and R values in muscle at 10 and 90minutes after slaughter. The meat quality was DFD in some pigs and PSE in others, regardless of usual or experimental slaughter.
A simultaneous epizootic of bovine tuberculosis and paratuberculosis broke out among cattle of the Japanese Brown breed 2 to 11years old in an area of Hokkaido. For 2years 50 cattle were condemned by tuberculosis and 19 by paratuberculosis. Mycobacterium bovis was isolated from 47/50 cattle and M. paratuberculosis from 13/19. Of the 50 cattle, 16 had macroscopical paratuberculous intestinal lesions. Of the 19 cattle, three had tuberculous lesions in some organs. Histopathological examination revealed that 4 cattle were involved in the dual infection with the 2 organisms.
Oxytetracycline (OTC) was fed continuously to chickens at various levelsf or 42days beginning at 29days old. Doxycycline (DOTC) was administered via drinking water for 5days beginning at 36days of age, when experimental birds came in contact with infected carrier chickens. Chickens were checked daily for clinicalo bservationsf or 35days thereafter. Protection was afforded by OTC alone at a level of 250g or more/ton of feed. The chickens were free from clinicals igns, g ross lesions, a nd Mycoplasma gallisepticum (MG) and were 100% serologicall My G-negative. Partial protection was produced by OTC at 125g/ton. One time 5-day administration of each OTC at 500g/ton of feed, DOTC at 50g/ton of water or tylosin at 500g/ton of water alone was not efficacious. Recovery of MG was reduced by continuousfeeding of OTC at 125g/ton of feed in combination with 5-day administration of DOTC at 50g/ton of water, as compared with feeding of OTC alone at 125g/ton. In conclusion, a combination of continuous feeding of OTC with intermittent administration of DOTC would be favorable in the field condition.
A 5-month-old Japanese Black cow was affectedw ith sudden anorexia and acute bloating, w hich did not respond to treatment. No bronchovesicular sounds were heard in the right lung area. The rumen catheter passed the chest-esophagus with difficulty. By exploratory laparotomy, a mass as large as a man's head in the thorax was palpated from the peritoneal cavityvia the diaphragm. Necropsy revealed an extended sac, with which the right pleural cavity was filled, in the cranial lobe of the right lung. This case was diagnosed as alveolar hypoplasia in which bronchioles failed to develop. The epithelium of the sac resembled that of the bronchiole.