Piglets with cleft palate were consecutively produced for ten months at a swine farm in Miyagi Prefecture. Some of them had cystic liver, gallbladder defect and arterial duct patency. One bore was associated with occurrence of the cleft palate, and chromosomal analysis revealed that piglets with cleft palate had a trisomy with chromosomal translocation.
Cows with displaced abomasum with severe clinical signs received a laparotomy and right omentopexy following the correction of the electrolyte imbalance by transfusion, and their clinical findings on the day of admission were compared between cases with good prognosis (6 cases) and those with poor prognosis. By their pathosis, the cows of poor prognosis were of infectious diseases (4 cases), fatty liver (5 cases), abomasal volvulus (4 cases) and the others (5 cases). The infectious disease and abomasal volvulus cases showed lower values of the blood lymphocyte count and serum potassium, as well as higher values of neutrophil count and blood urea nitorogen as compared with cases of good prognosis. The cases with abomasal volvulus showed lower values of serum chloride than those of good prognosis cases. The cases with fatty liver showed higher values of serum GOT and ketonuria as compared with good prognosis cases. These results indicated that the number and differential count of leukocytes, the levels of blood urea nitrogen and potassium might be reliable indicators of poor prognosis in cases with infectious diseases and abomasal volvulus. Higher values of GOT and ketonuria in cases with fatty liver might be indicative of poor prognosis.
Ultrasonography applied to the urinary bladder site of sows showed white and turbid image sometimes with strongly brightened floating particles in abnormal urine cases, while the image was echo-free in normal cases. The echogenic image of massive pus was observed in the bladder of abnormal urine cases. The brightened liquid image was dispersed after injection of boric acid solution into the bladder of abnormal urine cases. To collect the urine ultrasonography was useful for confirming the full-up of the bladder.
A prematurely born calf withweakness, dyssatasia and inability in suckling was euthanatised at 14 days of age. Histopathologically meningo-encephalomyelitis was seen, and a-hemolytic streptococcus was isolated purely from the brain and identified as Streptococcus suis type 10. The organism-specific antigen was demonstrated in the brain lesions.
From 1987 to 1990 a total of five cases of bovine viral diarrhea-mucosal disease (BVD-MD) were spontaneously observed within a limitted area of Oita Prefecture: one case of congenital abnormality with cerebellar hypoplasia; a newborn calf with blindness and feebleness; a calf and a dairy cow with mucosal signs; calves with respiratory signs, pyrexia and diarrhea. The cases with mucosal signs were from BVD-MD antibody highly positive farms.
Serum antibody response and its specificity were evaluated in puppies inoculated with canine corona virus (CCV) using neutralization test (NT), enzyme-linked immunosorbent, assay (ELISA) and Western blotting analysis. Correlation was high enough between NT and ELISA antibody titers. Cases which were negative in ELISA as well as in NT before inoculation, highly responded in Western blotting to three major viral proteins, VP145, VP50 and VP27, after CCV infection. Both NT-negative and positive (1:16) preinoculation sera reacted fairly with VP53 and VP56 after inoculation while weakly with VP27 and VP45. These data indicated that the early appearing antibodies to viral envelope antigens, VP27 and VP145, were specific and that the reaction to VP53 and 56 might be associated with the non-specific reaction in ELISA.
Nine dogs with flea allergic dermatitis diagnosed by immediate-type intradermal test, were injected with a self-made allergen, which was prepared from the whole flea by ultrasonication, filtration and incubation at 65°C. Nine dogs were subcutaneously injected with 1ml of a 1: 5, 000 (W/V) dilution of the allergen once a week. Six and 3 dogs received 12 or more and 4 injections, respectively. Six (66.7%) of 9 dogs responded to the treatment with a mean improvement rate of 73%(33-100%). Slight increases in indirect hemagglutination titers were demonstrated in 3 dogs, whereas 2 dogs showed decreases in Prausnits-Kustner titers after the treatment.
The serum levels of C-reactive protein (CRP) in apparently healthy dogs and those with various disorders or surgical traumas were determined quantitatively by enzyme-linked immunoabsorbent assay (ELISA) and semiquantitatively by slide reversed passive latex agglutination test (RPLA). The CRP values of 290 serum samples obtained by ELISA well agreed with those by RPLA (r=0.913). Semiquantification of CRP was possible by RPLA with serum, plasma, or whole blood. Canine CRP could not be determined by using the human-CRP measurement Kit.