A bovine disease of unknown etiology broke out in six prefectures of Japan, Fukuoka, Hiroshima, Nagasaki, Saga, Shimane, and Yamaguchi, in October and November, 1966. Its main symptoms consisted of transient high fever (40.0 to 42.0°C), polypnea, anorexia, loss of vigor, lacrimation, salivation, arthralgia, and tremor of muscles. About 7, 000 cattle were affected with this disease.
Strains Nagasaki and Yamaguchi were developed from defibrinated blood harvested from some affected cattle at the febrile stage. They were successfully subjected to serial passages in cattle. From the results of an infection prevention experiment with cattle, they were identified as strains of bovine epizootic fever virus.
A survey of antibodies against known viruses was conducted on acute-stage and convalescent sera collected from naturally infected cattle and pre- and post-inoculation sera from experimentally infected cattle. As a result, a significant increase in antibody titer was shown by all the sera tested against strain YHK of bovine epizootic fever virus (BHK 21-W2 cell-passaged virus), but not by any of the sera against bovine influenza Kaeishi virus, bovine diarrhea virus, bovine infections rhinotracheitis virus, the Fukuroi type of bovine adenovirus, or type 3 of parainfluenza virus. In conclusion, it was presumed that bovine epizootic fever virus might have played a principal role in the present outbreaks in Japan.
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