A cow had a lesion from which Actinomyces bovis and Corynebacterium pyogenes were isolated. It was subjected to a symptomatic treatment with a large amount of penicillin, which resulted in success in a short time. Histopathological examination revealed granulomatous inflammation. From these findings the cow was diagnosed as a case of actinomycosis.
Six Yorkshire piglets were purchased and raised on a pig farm in the suburbs of Hiroshima. They were housed in the same shed for 2months, when all of them were found to have tumors ranging from quail's egg to hen's egg in size and scattered on the skin of the whole body. Autopsy was conducted on them, except two, and revealed no other lesions than these tumors. Corynebacteriumpyogeneswas isolated from the pus of the suppurative foci of the 4 dissected piglets in pure culture, but not from blood, spleen, liver, kidney, or lung of any piglet.
Chicks bearing inherited maternal antibody against avian infectious bronchitis (AIB) were inoculated with live or inactivated AIB vaccine to study a method of immunization. Regardless of the type of vaccine, a higher neutralization titer was obtained at 49 to 56 days of age from chicks vaccinated twice at 4 and 28days of age than from those vaccinated twice at 4 and 14days of age or from those vaccinated thrice at 4, 14, and 28days of age. Older chicks bearing no antibody were inoculated once with live vaccine or twice with inactivated vaccine at an interval of 1 to 4 weeks. In them, the antibody titer increased to 2.2≤3 to 6weeks after the initial inoculation.
The present studies were carried out on the serum samples collected from a some big poultry farm in Tokachi district, Hokkaido, once a month throughout the period of June, 1968 to November, 1969, from the view point of their antibody responses and the influences of some drugs on them. The results are summarized as follows: 1) Development of Myocplasma gallisepticum (M. g.) antibody was observed in 30 to 71% of chicken flocks more than 3-month age and hold their titers through the whole observation period. On the contrary to this, Hemophilusgallinarum (H. g.) antibody persisted in low percentage at first, but increased suddenly to about 70% at the age of 9months and after. Egg production was influenced in a chicken flock of high positive ratio ofM. g. antibody. However, such co-relation was never observed from a view point ofH. g. antibody. During the whole observation period, no clinical case of respiratory diseases was noticed. 2) Administration of tylosin tartrate with the drinking water into the young chicken flock which have heavy incidences ofM. g. antibody caused reduction in the antibody titers. The same effect was also observed temporarily in adult flocks by intramuscular inoculation of this antibiotics. 3) Feeding a young or adult chicken flock with diet contained tylosin phosphate only or mixture of Furamizole on the ratio of one week per month suppressed the development ofM. g. antibody, as compared with the control group. However, this finding was not observed in the flocks which were highly contaminated withM. g. infections. In the administration group of spiramycin with diet or in the muscular inoculation group of tylosin tartrate, the suppressive effects on the development ofH.g. antibody were not determined from the result of the present study. 4) In natural case ofM. g. infection, the young chicken showed high agglutinating antibody titers in compared with the titers of hemagglutinationinhibiting antibody, but in the adult one many hens indicated higher hemagglutination-inhibiting antibody titers than that of agglutinating antibody.