Ampicillin, -or 6 [D (-)-α-aminophenylacetamido] penicillinic acid, was evaluated for. curative effect on bacterial diseases in 54 dogs and 14 cats. An attempt was made to isolate causative organisms and examine them fbr susceptibility to some antimicrobial agents. Ampicillin was injected once a day intramuscularly at a dose of 5-10mg/kg or administergd. orally at a dose of 25-40mg/kg. The results obtained are summarized as follows. 1. Of the 68 animals tested, 54 recovered well by the treatment of ampicillin. Good results were obtained from most of the animals affected with alimentary and urinary tract infections and from 80% or more of the animals suffering from suppurative dermatitides and respiratory tract infections. Only poor responses were observed in most of the cases of abscess, otitis and endometritis. 2. Various species of Staphylococcus, Proteus, Escherichia, Streptococcus, and Pseudoxnonas were isolated from 32 of 39 animals examined prior to the treatment. Staphylococci and Gram-negative bacilli were frequently isolated from suppurative dermatitides and respiratory tract infections, respectively. 3. Ampicillin was significantly effective against about 90% of the strains, inhibiting the growth of both Gram-negative and positive organisms. Its antimicrobial activity was similar to that of tetracycline or chloramphenicol.
Cardiotomy under block of venous circulation was performed on 30 experimental dogs. The limit of safety for circulatory block was confirmed to be about one minute. The rate of successful detection of filariae was 76% on the average, which was higher than that obtained by the K technique for the removal of heartworms. When the cardiotomy was applied clinically to 26 dogs, the survival rate of the dogs was 84.6% and the number of worms dctected was 5-89, or 45 on the average, per capita. The time of circulatory block was 35-120 seconds, or 69 seconds on the average. These results were anticipated from those obtained from the experimental dogs.
A mixed Marek's disease and Newcastle disease live virus vaccine (MNV) was prepared from herpes virus of turkeys and strain B, of Newcastle disease virus, and examined for safety, immunogenicity, and cohabitation infectivity. The results obtained are as follows. 1. MNV was safe for SPF and commercial dayold chicks. 2. Both vaccine virus did not interfere with each other. MNV showed the same immune effect as Marek's disease vaccine and Newcastle disease vaccine inoculated independently. 3. MNV revealed a little better results when inoculated intraperitoneally than when inoculated intramuscularly or subcutaneously. 4. MNV exerted no influence upon Newcastle disease live virus vaccine inoculated later. 5. Infection with herpes virus of turkeys was found in one of the birds placed in the same house with those inoculated with MNV immediately after inoculation. Infection with Newcastle disease virus was demonstrated in none of these birds.