Primary hypothyroidism was diagnosed in a 4-year-old golden retriever bitch with a disturbance in gait as a chief complaint. This bitch showed bilateral alopecia, hyperpigmentation, thicking and wrinkling of the skin, seborrhea, and myxedema-like edematous skin. Anemia, hypercholesteremia, and low values of serum thyroxine (0.4 μg/dl) and triiodothyronine-binding capacity index (0.88) were revealed. Serum protein-binding iodine was normal (2.8 μg/dl), as compared with that in 15 normal dogs (3.3±1.58 μg/dl). The values of these three thyroid function tests (T4, TBC index and PBI) showed no significant changes 24 hours after TSH (5-10 IU) stimulation, as compared with those before stimulation. In contrast, 5 normal dogs exhibited a significant increase (averaging 2.8 times) in PBI response to TSH stimulation. The spread of the iodine preparation on the skin surface boosted the PBI value (averaging 5.3 times). Treatment consisted of sodium L-tetraiodothyronine replacement at a dosage of 5-7.5 μg/kg, once a day. Clinical response was good to hormone therapy within 1 month. The atrophic thyroid gland was characterized by the severe destruction of follicles and replacement of most of the thyroid by fibrous connective tissue.
A helminthological survey was made on 105 stray domestic cats autopsied in Kanagawa Prefecture, Japan, over a period from October, 1981 to Nobember, 1982. The cats were classified into two groups according to the conditions of tooth exchange. Group A consisted of 53 cats more than 3-4 months old and group B of 52 cats younger than these. Parasitic helminths were detected from 66.0% of group A and 63.5% of group B. The infection rates of each species were as follows: Spirometra erinacei was detected from the small intestine of 17.0% and 1.9%, Dipylidium caninum from that of 20.8% and 13.2%, Taenia taeniaeformis from that of 1.9% and none, Strongyloides planiceps from that of 1.9% and 5.8%, Toxocara cati from that of 24.5% and 57.7%, Ancylostoma tubaeforme from that of 35.9% and none, Capillariasp. from the bladder of 3.8% and none, Physalopterasp. from the stomach of 1.9% and none, and Dirofilaria immitis from the heart of 1.9% and none of group A and B, respectively.
Healthy bee colonies were inoculated with Ascosphaera apisM S 7911 isolated by Mabuchi in 1979. Many larvae became mummified and chalky white 3-5 days after inoculation. Larvae 4 days old were the most sensitive. The organism was isolated from them. A 1: 800 dilution of disinfectant and 0.5% sodium propionic acid were effective for the prevention of infection.
By immunodiffusion analysis low molecular weight (LMW) IgM was detected in the sera of chickens on two farms, A and G. to make the differential diagnosis of Marek's disease (MD) and avian lymphoid leukosis (ALL) in the field. The diagnosis of MD was made in some chickens on farm A because of the absence of tumors in the bursaof Fabricius (BF) and LMW IgM in serum and the presence of histopathological changes characteristic of MD. The diagnosis of ALL was made in some chickens on farm G because of the presence of LMW IgM in serum, distinct lymphoid tumors in the BF, and microscopic tissue changes associated with ALL. It was concluded that ALL with BF tumors had produced LMW IgM, and MD no LMW IgM.
Samples of milk from 673 cows with normal udders were examined in Saitama Prefecture. Yeasts were detected in 36 udders (5.3%). They belonged to the genus Candida (19 strains), Trichosporon (3.) Rhodotorula (5), and Troulopsis (4). Seven of them were unidentified. The number of organisms ranged from 10 to 3, 300 per ml. The isolated yeasts were the most sensitive in vitro to Clotrimazole of 8 antimycotics examined.
Incomplete atrioventricular canal malformation with subaortic stenosis was observed in a 9-month-old Large Yorkshire female pig. The pig showed poor growth, severe weakness, hyperpnea, and tachycardia. A week after the first examination, it was destroyed with the owner's agreement for dissection. An autopsy revealed an atrial septal defect of the ostium primum about 8 mm in diameter. A mitral cleft was present in the central portion of the anterior leaflet. A tricuspid sac was formed at the site of the membranous septum. The inflow tract of the left ventricle was clearly shorter than the outflow tract. The basal portion of the ventricular septum had a scooped-out and concave appearance. The outflow tract of the left ventricle was narrowed and elongated by “gooseneck” formation. Subaortic stenosis due to fibrous tissue proliferation was present immediately below the aortic valve with the lumen reduced to about 7mm in diameter. The edge of the mitral cleft was thickened, indicating that mitral insufficiency of a mild to moderate degree might have been present. A part of the chordae tendineae of the tricuspid anterior leaflet was inserted into the septomarginal band which was of high take-off.