Ten dairy cows secreting alcohol-unstable milk (CAU) and ten secreting normal milk (CN) were examined for iodine combining with protein in the serum. The values obtained were 3.1±40.78 γ/dl in CAU and 3.60±0.93 γ/dl in CN, showing no significant difference between them.
Calcium and inorganic phosphorus, when estimated in the same serums as usedabove, were 9.21±0.72 and 5.46±0.85mg/dl in CAU and 9.07±0.83 and 5.54±0.68 mg/dl in CN, respectively. No significant differences were recognized between these values.
In conclusion, it is assumed that no abnormality in the functions of the thyroid and parathyroid glands exercised influence directly upon alcohol-unstable milk.

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A dose of 50 metacercariae of Fasciola gigantica, from which had been removed outer cysts, was injected to rabbits in the longissimus muscle and in the subcutaneous tissue of the back and thigh. Two rabbits receiving subcutaneous injection at the thigh passed without manifesting clinical symptoms. Eight of nine animals receiving subcutaneous or intramuscular injection at the back exhibited aralysis in the fore and hind quarters and ischuria 28 to 65 days after injection and succumbed or were moribund 1 to 11 days later.
These symptoms were observed on 4 rabbits of 54 given 20 to 50 metacercariae each per os 12 to 71 days before.
Gross changes in rabbits suffering from paralysis in the fore and hind quarters consisted of remarkable hemorrhage, cell infiltration, and degenerative changes in the muscle and subcutaneous tissue in which metacercariae had been injected. Hemorrhage in the spinal cavity and hemorrhage and cavitation in the parenchyma were also seen.
In the rabbits infected by the parenteral route, young larvae with inhibited growth were found in lesions of the muscle and the subdural portion of the spinal cord. They were detected also from the liver of such animals as autopsied 46 or more days after infection.
One to three young larvae were found in each spinal cord in 7 of 12 rabbits exhibiting paralysis in the hind quarters. It has been made clear that hind-quarters paralysis and ischuria were caused by aberration of liver flukes in the spinal cord.

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Immune serum was prepared from goats against rabies fixed virus. It was purified and concentrated by Pope's modification of the salting out method with ammonium sulfate. As a result, the yield of purified and concentrated serum was about 50 to 70 per cent of the original immune serum. In general, the quality of immune serum was considerably improved by this treatment. A rather high effect of preventing clinical symptoms from appearing was recognized on animals injected with purified serum within 2 to 10 hours after virus had been inoculated. Survival period was increased by 24 to 72 hours in animals injected with purified serum within about 20 hours after virus inoculation.
A further advance in studies on the present subject may make it possible to develop any practical method for the prevention of rabies with immune serum.

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