Seven, 54, and 10 embryos were transferred to solution N2 at-20, -30, and-36°C, respectively, and then thawed rapidly at-360°Cfmin. The storage medium used were DB+20-50% BS or FCS in case of-20 and-30°C, and Ringer's solution +30%BS in case of-36°C. They contained 1.0M and 1.25M glycerol, respectivery. Of the 7 embryos in case of-20°C, only one survived and the others were regarded as degeneratire ones at 48 hours of culture. Of the 54 and the 10 embryos in case of-30 an-36°C, 25 and 5 survived, suffering from limited damage at 2 and 24 hours of culture, respectively. Cows became pregnant when 3 of the 20 and 2 of the 5 embryos in case of-30 and-36°C, respectively, were transplanted to them.
When examined by electrocardiography a 6-year-old male Boxer dog showed a first-degree atrioventricular block, ventricular premature beats, and ventricular tachycardia. In it, phonocardiography, radiography, and blood examination suggested myocardial disease. Echocardiography reveal that the ratio of the thickness of the ventricular septum to that of the posterior left ventricular free wall was 1.5. Mitral valve echo indicated an anterior systolic movement. These echocardiographic findings were considered specific enough to be important parameters in making a diagnosis of canine hypertrophic cardiomyopathy.
In April and October, 1981, attempts were made to isolate Treponema hyodysenteriae from 147 swine fecal samples collected on 74 farms in 31 prefectures. As a result, T. hyodysenteriae was isolated from 57 samples (38.8%) from 35 farms in 19 prefectures. When classified by the district, T. hyodysenteriae was mostly isolated in samples from Kyushu. When classified by age, T. hyodysenteriae was found in samples about 5 or 6 manths after inoculation. When classified by the type of feces, T. hyodysenteriae was not isolated from normal or soft feces, but from semisolid and watery feces. The number of T. hyodysenteriae organisms isolated was larger in watery feces with occult blood than in any other type of feces. On the other hand, β weakly-hemolytic Treponema was isolated from 6 samples (4.1%) collected on 4 farms. The in vitro sensitivity test was conducted on 90 strains of T. hyodysenteriae (obtained from 57 samples) and 6 strains of β weakly-hemolytic Treponema, with the DJ70 strain as a control, to 4 compounds, carbadox, olaquindox, tiamulin, and tylosin. As a result, all the strains of T. hyodysenteriae, including the control, exhibited marked sensitivity to carbadox and high sensitivity to olaquindox and tiamulin, but showed two-peaked sensitivity to Tylosin. Besides, β weakly-hemolytic Treponema exhibited the same sensitivity pattern as T. hyodysenteriae, except that it had no strains resistant to Tylosin.
Four cows slaughtered as infected with tuberculosis were examined histopathologically and bacteriologically in Saitama Prefecture in 1980. All of them were non-visible lesion reactors, but Mycobacterium kansasii were isolated from one of them. This organism was first isolated from cows in Japan. A stronger reaction to mammalian tuberculin than to avian tuberculin was presented by guinea pigs sensitized with M. kansasii. Guinea pigs sensitized with M. kansasii and M. tuberculosis showed the same reaction to mammalian tuberculin as to avian tuberculin. Therefore, a cross-reaction between both organisms was suggested.
Granulomatous (histiocytic ulcerative) colitisis a well-known disease in Boxer dogs, although it has not been reported in Japan. The present case was an 11-month-old male inbred Boxer with illness for more than 8 months, which had a history of intermittent excretion of soft stool with a little blood. Long-term symptomatic treatment with antibiotics was ineffective, and euthanasia performed. Necropsy revealed the diffusely thickened colonic wall, multiple ulcerative colonic mucosa, and enlarged mesenteric lymph nodes. These lesions were characterized by extensive infiltration of histiocytes containing abundant PAS-positive material.