Journal of the Japan Veterinary Medical Association

[title in Japanese]

[in Japanese]

2003 Volume 56 Issue 11 Pages 683-684
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.683

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Trends in Antimicrobial agents for Veterinary Use and Control Measures of Antimicrobial Resistance

Yutaka TAMURA

2003 Volume 56 Issue 11 Pages 685-691
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.685

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[title in Japanese]

[in Japanese]

2003 Volume 56 Issue 11 Pages 695-696
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.695

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Antibody Tests and Isolation of the Cytopathogenic Bovine Viral Diarrhea Virus

Toshiya SAITO, Osamu YAMAGUCHI, Katsuhiko FUKAI

2003 Volume 56 Issue 11 Pages 717-721
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.717

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Antibody tests and isolation of the bovine diarrhea virus (BVDV) have been performed on primary bovine fetal muscular-cell cultures. The cytopathogenic effect (CPE) was the index in the case of cytopathogenic viruses. The interference method was employed in dealing with non-cytopathogenic types, which demonstrate no CPE. In this study, we made use of the characteristic of bovine kidney cell line (MDBK-SY) to be induced the CPE by both CP and NCP viruses and examined the possibility of a new BVDV assay method. This new assay was used in isolating 4 BVDVs from field specimens. Previous assays had identified one of these strains as CP BVDV and the other as NCP BVDV. Both previous assays and the new assay were used to determine the neutralization antibody titers to Nose, No.12, and KS86-1 NCP in serum specimens. The neutralization antibody titers to those strains determined by the new assay correlated significantly with those determined by previous assays. These results suggest that the new assay established in this study is a useful system for BVDV investigation.

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Reasons for Anestrus in Japanese Brown Cows Fed Soybean Waste

Masami HAMADA, Kunijiro YOSHITAMA

2003 Volume 56 Issue 11 Pages 722-727
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.722

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The residue of sorting, soybean waste is a mixture of numerous plant fragments and defective seeds. Japanese Brown cows fed this waste demonstrated anestrus. To investigate the reasons for this phenomenon, reverse-phase HPLC was used to measure plant-estrogen isoflavonoids in the feed. These measurements showed that, in addition to daidzein and genistein, soybean waste contains a great deal of formononetin (76.48μg/g) and coumestrol (89.01μg/g).Coumestro1, daidzein, and equol were detected in the serum of anestrous cows. After amounts of soybean waste in the diet were reduced, estrus occurred again within a month. At this time, no isoflavonoids were detected in the blood. These results indicate the possibility that isoflavonoids in soybean waste may have caused anestrus in the animals examined.

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Incremental Administration of o, p'-DDD to Four Dogs with Spontaneous Hyperadrenocorticism

Toshinori SAKO, Hiroko FUKUTA, Hisashi MIZUTANI, Hidekazu KOYAMA, Hisa ...

2003 Volume 56 Issue 11 Pages 733-735
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.733

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Four dogs with spontaneous hyperadrenocorticism were treated with incremental administrations of o, p'-DDD. The initial dosage was 5mg/kg. Subsequent doses were administered at intervals of 3 to 5 days with a stepwise increase of 5mg/kg. After the dosage level reached 25 mg/kg, doses were raised at 2 to 3mg/kg increments. When such clinical signs as polyposia and polyuria had improved, the adrenocorticotropic hormone (ACTH) stimulation test confirmed the effectiveness of the therapy. The goal of treatment with o, p'-DDD was to achieve an ACTH stimulation result suggesting a normal range of responses. Possibly gradual o, p'-DDD dosage increases made it possible to achieve optimum individual dosages that resulted in high efficacy.

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Traumatic Ischemic Myelopathy in a Cat

Yoshiharu OKAMOTO, Katsuyuki MIYATAKE, Takehito MORITA, Masumi SAWADA, ...

2003 Volume 56 Issue 11 Pages 736-738
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.736

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A 4-month-old female Siamese cat weighing 1.8 kg was found in a state of collapse. Neurological examinations revealed paraplegia of both hind limbs and loss of reflexes in the patellar tendon and anal sphincter muscle. Lumber myelograph revealed an irregular, marbled pattern in the image of the lumbar spinal cord, though no abnormal X-ray findings were observed. Necropsy showed fresh hemorrhage in the subcutaneous tissues of the inner thigh, the pelvic cavity, and the right abdominal wall. In addition, the lumbar spinal cord was swollen. Histological examination revealed acute ischemic neuronal necrosis and neuropil edema in the spinalcord gray matter from L2 to the end of the sacrum. On the basis of these findings, the case was diagnosed as traumatic ischemic myelopathy.

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Direct and Rapid Detection of Bacillus anthracis DNA Added to Cattle Blood and Meat

Wataru MATSUNE

2003 Volume 56 Issue 11 Pages 741-744
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.741

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Bacillus anthracis Pasteur II cells were added to cattle blood and meat to investigate a direct, rapid method for detecting DNA by means of nested PCR targeting the PA and capA genes. In preparing the samples for nested PCR, a cell disrupter and spin columns proved effective. It was possible to detect target DNA amplicons from 1 ml of blood containing 10⁴ cfu and from 1 g of meat containing 10⁵ cfu. DNA detection required only 4 hours. In association with such bacteriological methods as the ascoli test, the phage test, and bacterial culture, this simple, speedy system can be useful for diagnosis of cattle anthrax.

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Prevalence and Characteristics of Enterohemorrhagic Escherichia Coli O157 Isolated from Cattle at Slaughterhouses in Hiroshima

Hiroaki SAKAGUCHI, Akemi KYOUZUKA, Minori KODAMA, Kouzou SAIKI, Koji Y ...

2003 Volume 56 Issue 11 Pages 745-749
Published: November 20, 2003
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.56.745

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Inspections of randomly collected cecal contents of 675 head of cattle from about 40, 000 animals processed at slaughterhouses in Hiroshima between April 1999 and March 2002 detected enterohemorrhagic Escherichia coli (EHEC) O157 in 35 animals. The overall carrier rate was 5.2% and tended to be high among Japanese Black cattle. The strain was detected only in animals less than 3 years of age. Detection rates were higher in warm summer months. All detected EHEC O157 serotypes were H7, and VT2-positive strains were in the majority (54.3%). Among cattle on farms where EHEC O157 was continually detected, drug susceptibility and VT type were identical. RAPD profiles of the strain showed patterns differing as a result of several kinds of primers.

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