Piglets suffering from diarrhea, as well as normal healthy piglets, wereadministered forcibly with 50mg/kg of piromidic acid (PA) daily or with 25 mg/kg of PA twice a day by the oral route for 3 days. In them, the fecal level of PA 15-24 hours after the end of treatment was high enough (3-15 times as high as MIC) to inhibit the multiplication of Escherichia coli. When diarrheal piglets were administered orally by force with 25 mg/kg of PA twice a day for 3-7 days, they showed a rather high rate, or 78.8%, of effective treatment. The rate of gain inbody weight was improved in piglets treated successfully with PA.
On a pasture in the southern part of Kagoshima Prefecture 10 calves 6-33 days old were affected with diarrhea. A virus was isolated from the feces of three of these calves. It was identified as bovine rotavirus (BRV) by electron microscopy and the fluorescent antibody technique. Antibody against BRV (Lincoln strain) showed a significant increase in titer one month after the onset of diarrhea. The colibacillary colony count increased to 108-409/g in the diarrheal feces, suggesting a secondary participation of colibacilli in the etiology of the diarrhea.
Bovine subclinical mastitis was treated with a single oral dose of 7.5mg/kg of levamisole in 53 cows with 100 quarters affected. The treatment was successful in 58.9%(33 of 56 quarters). Milk production was markedly improved 3-4 months after dosage. The treatment of dry quarters with this drug was successful in more than 75%, regardless of under infusion of antibiotics. The milk cell count was remarkably reduced 2-3 days after dosage, but increased distinctly 4-7 days after dosage. The bacterial count of milk showed a clearly significant reduction 4-11. days after dosage. In peripheral blood, there was an increase in lymphocyte count and percentage of T-cells 7 days after treatment. With its enhancing effect on cell-mediated immune responses, levamisole seemed to be of special value in the treatment of bovine subclinical mastitis.
Chicks originated from SPF eggs were inoculated with Salmonella typhimurium and raised with intact chicks. One week after inoculation the organism was detected from the cloaca of 92 100% of all the chicks, including the latter. Ten chicks died of septicemia caused by the organism. Ten chickens uninoculated and surviving the contact infection were inoculated with S. typhimurium at 259 days of age. The organism was detected from the cloaca of seven of them 3 weeks or later after inoculation. It was discharged for a short time in a count of 5.5×103 per gram of feces. Autopsy performed 48 days after inoculation revealed the presence of S. typhimurium in the cecum of 7 birds, the rectum of one bird, and the parenchymatous organs of no birds, in addition to the cloaca. Of 172 eggs laid by the hens for 11 days, 23 harbored this organism in both egg yolk and shell. Bacterial isolation was negative in 139 eggs laid for the subsequent 20 days.
Nine adult quails were affected with lymphomatotic disease. In them, the liver exhibited an enlargement with whitish foci. The spleen and proventriculus appeared grossly to be enlarged. The small intestine showed whitish thickening. The ovary was atrophic. Histopathological changes were present in these organs, lungs, kidneys, heart, pancreas, and sciatic nerves. They were characterized by diffuse invasive masses of lymphoid cells of various sizes. The proliferative focus of the liver exhibited an increase in argyrophil fibers. Marek's disease (MD)-specific antigen was found at the feather tips of all the birds and MD-specific antibody in the serum of 2 birds.
Ovarian teratoma was found in the right ovary of a 2-year-old cow. The ovary was 16.0×15.0×11.0cm in size with a large follicular cyst containing 1, 250ml of follicullar fluid. The teratoma was very hard and 7.5×3.5×3.0cm in size. It had various kinds of tissues, such as cartilage, pulmonary, bronchial and gland-like tissue, epidermis, and hair, in irregular arrangement. The left ovary was normal.