A total of 236 dogs of 20 breeds, in which a positive diagnosis of filariasis had been made at the out-patient clinic, were treated with dichlorophenarsine hydrochloride (0.75mg. AS/kg daily for two consecutive days).
1. Emesia, nausea, and shock occurred several to 30 minutes after injection, in 51.6 and 36.9 per cent of the medicated dogs after the first and second dose were given, respectively. Emesia and nausea were transient. Shock appeared in 1.2 per cent of the dogs, the two injections combined, and brought about a fatality rate of 0.8 per cent.
2. This treatment caused no liver dysfunction nor any toxic symptom related to it.
3. One or two weeks after treatment worms were expelled and induced embolism of the pulmonary artery in 28.9 per cent of the treated dogs, 7.6 per cent of which was fatal. The occurrence of the embolism and that of fatal cases increased in frequency parallel with the advance in course of disease.
4. Deaths from embolism of the pulmonary artery occurred more frequently in a four-month period beginning with November than in the other months. During that period 20.8 per cent of the medicated dogs succumbed.
5. Within two months after treatment, death rate was 1.9 per cent among dogs of stage I of disease, 5.6 per cent among those of stage II, 7.0 per cent among those of stage III, and 53.3 per cent among those of stage IV. From these results, it has become possible to predict the rate of risk attending the treatment of particular dogs when these dogs can be classified by the stage of filariasis.

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The so-called slimy (neto in Japanese) phenomenon is induced in sausage by the multiplication of bacteria attached to the surface of the product. In part I of this series of studies, it was clarified that the slimy substance was a mass of propagated bacteria. In this investigation, the bacterial flora constituting the slimy substance was studied with special reference to its relations to the temperature of storage of sausage.
1. The bacterial flora constituting the slimy substance varied according to the temperature at which the slimy phenomenon occurred.
2. The slimy substance appearing at 37°C was composed almost exclusively of Gram-positive organisms, particularly those of Corynebacteriaceae, Gaffkya, and Staphylococcus.
3. The bacterial flora of the slimy substance which was produced at 20°C consisted mostly of Gram-positive organism. Gram-negative ones, however, were detected from it at a rather high frequency. The Gram-positive ones detected were composed of Corynebacteriaceae, unidentified bacilli, Gaffkya, Staphylococcus, and Lactobacillus, arranged in the decreasing order. The Gram-negative ones found were mostly of Alcaligenes, Pseudomonas, and unidentified bacilli.
4. A majority of the strains detected from the slimy substance appearing at 4°C were of Gram-negative organisms. Those strains were mostly of Pseudomonas, Alcaligenes, and unidentified Grampositive bacilli. Few of them were of some other species. A decrease in Gram-positive organisms was pronounced.
5. Most of the organisms derived from the slimy substance showed sufficient growth at 20 to 37°C were thermolabile, had a strong resistance to sodium chloride, and could grow even in strongly alkaline culture media.

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