Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 65 , Issue 9
Showing 1-7 articles out of 7 articles from the selected issue
Farm Animal Medicine and Animal Health
  • Takeshi OSAWA
    Type: Review
    2012 Volume 65 Issue 9 Pages 673-681
    Published: September 20, 2012
    Released: May 26, 2017
    JOURNALS FREE ACCESS
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  • Masaaki NAKAMURA, Takeshi KOYAMA, Yoshitaka MATSUI, Tsunao HIRAI, Akir ...
    Type: Original Article
    2012 Volume 65 Issue 9 Pages 682-688
    Published: September 20, 2012
    Released: May 26, 2017
    JOURNALS FREE ACCESS
    Hemoglobin Binding Assay (HBA), one of the methods of bovine serum haptoglobin (Hp) analysis, was modified to shorten the reaction time. The values of bovine serum Hp determined by modified HBA highly correlated with those determined by single radial immunodiffusion assay, and the assay reproducibility (intra- and inter-assay) was acceptable. In healthy postpartum cows, the concentration of serum Hp was elevated until 3 days after parturition and subsequently decreased. The values of the mean, median, and 95th percentile in healthy cows (6-16 weeks after parturition, n = 754) were 110, 98, and 170 μg/ml, respectively. The median value in healthy heifers (n = 41) was 88 μg/ml (mean 91 μg/ml). The levels of serum Hp in cows suffering from post parturient mastitis and a retained placenta were significantly higher than those in healthy cows (1 and 3 days after parturition). Serum Hp was significantly high in cows with acute mastitis or clinical mastitis, compared to those in healthy cows. These results suggest that the modified HBA could be a useful method for diagnosis in postpartum cows.
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  • Masaru SUGAWARA, Yuichi MATSUMOTO, Masahiko KABEYA, Hidetaka ONISHI, K ...
    Type: Original Article
    2012 Volume 65 Issue 9 Pages 689-693
    Published: September 20, 2012
    Released: May 26, 2017
    JOURNALS FREE ACCESS
    In this study, we assessed six cases of extraintestinal pathogenic Escherichia coli (ExPEC) infection in Japanese black calves and used PCR analysis to detect the virulence-associated genes of the ExPEC isolates obtained from the internal organs of each calf. The clinical signs in the calves included ananastasia, opacity eyes, and respiratory disorder. Histological analysis confirmed the presence of bronchopneumonia, multifocal necrosis,and centrilobular necrosis in the liver, purulent meningitis, and disseminated intravascular coagulation. The virulence-associated genes of the ExPEC isolates encoded toxins (cnf2 and cdt III ), adhesins (F17A and afa8), and siderophores (iutA, fyuA, irp1, and irp2). These results indicated that the virulence-associated genes of these E. coli strains may cause ExPEC infection in calves.
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  • Hajime KATO, Masatsugu SUGIYAMA, Motoki SAKUMA, Osamu YONEYAMA, Reiich ...
    Type: Short Communication
    2012 Volume 65 Issue 9 Pages 694-697
    Published: September 20, 2012
    Released: May 26, 2017
    JOURNALS FREE ACCESS
    We examined the antibody response of young Holstein breeding calves to Mannheimia haemolytica (Mh) vaccine containing inactivated capsular antigen and leukotoxin (LKT). Twenty calves that had been raised on a dairy farm in the Nemuro District of Hokkaido, Japan, and that had received adequate colostrum, were allocated to four groups that were vaccinated under different schedules. Group A (5 calves) received no injection of Mh vaccine; Group B (5) received a single injection at age 1 month; Group C (5) received a single injection at 5 months; and Group D (5) received two injections, the first at 1 month and the second at 5 months. After weaning, antibody titers against whole cell antigen in Group B were significantly higher than those in Groups A and C. There was no significant difference between Group B and D. These results suggest that a single,early vaccination could ameliorate Mh-associated respiratory disease in calves at weaning and during transportation.
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  • Manabu YAMADA, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Type: Data and Information
    2012 Volume 65 Issue 9 Pages 698-700
    Published: September 20, 2012
    Released: May 26, 2017
    JOURNALS FREE ACCESS
    Download PDF (593K)
Small Animal Medicine
Veterinary Public Health, Wildlife and Environmental Conservation
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