We investigated the potential of substituting the aqueous humor of sheep for blood by comparing the biochemical values of uremia and icterus in slaughter inspection. Using plasma and aqueous humor for 61 Suffolk sheep (Ovis aries ) brought to the slaughterhouse, we performed both correlation and regression analyses by measuring each biochemical value (urea nitrogen, creatinine, total bilirubin). The plasma measurements were higher than the aqueous humor measurements. The high coefficient of correlation and the decision coefficient were detected in the urea nitrogen values for both the wether and ewe groups. As for the creatinine values, the high coefficient of correlation and the decision coefficient were detected only in the wether group. No correlation was detected in the total bilirubin measurements. In conclusion, we would be able to estimate plasma biochemical values using aqueous humor and evaluate uremia of sheep even if blood is not available. However, it is difficult to achieve highly precise estimates of creatinine in females.