On October, 1971, a 4-month-old male Himalayan cat died of feline infectious peritonitis after suffering rom severe ascites, dyspnea, and anorexia for 11 days Congenital pericardiac diaphragmatic hernia was evealed by postmortem examination. The hernial ontents in the pericardiac space, were malformed hepatic tissue having developed from the left medial lobe of the liver. Fibrous inflammatory lesions were recognized on the serous membrane. The pericardium was enlarged by the hernial contents. The heart was compressed leftward and twisted.
Immunogenicity and safety of Newcastle disease (ND) infectious bronchitis (IB) inactivated combined vaccine (the combined vaccine for short) were studied in chickens 4 to 40 days old. ND hemagglutinationinhibition (HI) titer was 16 to 50 in a group of birds inoculated with the combined vaccine. It was 32 to 56 in a group of birds inoculated with ND vaccine Namely, there was no difference in titer between the two groups. IB neutralization (NT) titers were 3.0 to 4.0 in a group inoculated with the combined vaccine and a group inoculated with IB vaccine. There was no difference in titer between the two groups. In the groups inoculated with the combined vaccine, ND HI titer reached a maximum approximately 4 weeks postvaccination and then dropped, but IB NT titer maintained a high level until 8 weeks postvaccination. A rise in ND HI titer and IB NT titer was insufficient in birds 4 to 10 days old because of the presence of maternal antibodies. In these trials, no death or any noticeable clinical symptom occurred to the birds inoculated with the combined vaccine. Both ND HI titer and IB NT titer showed a satisfactory rise.
The skin was examined pathologically in 18 field cases and 1 experimental case of Marek's disease of chickens. Grossly visible skin tumors were detected in 6 cases (31.6%). Only two of these 6 birds had more than 10 skin tumors, whereas the other four had 1 to 10 tumors. In cutaneous sections, neoplastic lymphoid aggregates were observed in 11 cases (37.9%). Most of them were present adjacent to feather follicles. They frequently coalesced and expanded into interfollicular or subcutaneous tissues. Dermal nerves were usually infiltrated with lymphoid cells. In stained preparations of nine of 15 cases examined, eosinophilic intranuclear and cytoplasmic inclusions were demonstrated in the feather-follicle epithelium. Numerous naked capsids and enveloped particles of herpes-type virus were easily detected in the superficial layers of the follicular epithelium of all 3 birds examined by the electron microscopic technique of thin sectioning. In four of 6 cases examined by the agar gel precipitin test, specific precipitating antigens were detected in extracts prepared from the skin. There was an apparently positive relationship between the cutaneous neoplastic lymphoid lesion and either inclusions or precipitating antigens. In a few cases, however, no utaneous tumorous changes could be observed despite the presence of inclusions or viral particles in the feather-follicle epithelium. These observations suggest the possibility that infectious Marek's disease virus might be produced in and released from the skin, independently of cutaneous tumor.
In the past 10 years, ovarian tumors were found in 5 cows and 2 sows at the veterinary diagnostic laboratory of the Tottori University. The cows involved were 4 to 12 years old and two of them showed metastasis. The sows involved were multiparous Berkshire and Yorkshire and affected with hemangioma. A Holstein had granulosa- and theca-cell tumor of malignant type accompanied by luxuriant cartilage and cyst formation. Another Holstein and 2 indigenous Japanese Black cows bore granulosa-cell tumor. An indigenous Japanese Black cow showed soft fibroma.