Lipid peroxide concentration in dog foods A, B, C and D available on the market was determined using 2-thiobarbituric acid (TBA). The mean concentrations were 29.2, 6.1, 9.8 and 3.1 nmol/g in dog foods A, B, C and D, respectively. These dog foods were then kept in a refrigerator (4°C) or a darkroom, and exposed to a fluorescent light or the sun, and changes in the lipid peroxide concentration were examined in each of the dog foods for 30 days. The concentrations in dog foods exposed to a original light and the sun increased to 350-630% and 700-2100% of the original value, respectively, after 30 days. The dog foods kept in a refrigerator or a darkroom, however, showed only a slight increase in concentration. To inhibit the increase in lipid peroxide concentration, it seems desirable to keep dog foods in a refrigerator or a darkroom.

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In order to recognize the relationship of bile secretion and related blood levels after oral administration of fat soluble vitamins in calves, changes in serum tocopherol and vitamin A levels were examined in two groups, one administered only vitamin AD₃E (AD₃E group) and another receiving both vitamin AD₃E and cholagogue (cohlagogue group).
In the calves who were administered 1, 000 IU of vitamin E orally, increased serum tocopherol levels after the administration continued for 8 to 24 hours in the AD₃E group and for 8 to 48 hours in the cholagogue group. In the calves orally administered 2, 000 IU of vitamin E an increase of serum tocopherol levels continued for 8 to 24 hours in the AD₃E group and for 8 to 72 hours in the cholagogue group. In addition, the increased rate of serum tocopherol level was higher in the cholagogue group than in the AD₃E group. In the calves orally administered 2, 500, 000 IU of vitamin A the rate increase of serum retinyl palmitate level was higher in the cholagogue group than in the AD₃E group.
From the findings described above, it was considered that cholagogue is effective for promotion of intestinal absorption of vitamin E and A administered orally in calves.

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An outbreak of European foulbrood disease was observed at an apiary in Ibaraki Prefecture in May, 1987.
Of 23 hives that had been bread, three hives were affected. Eleven dead larvae were collected from one diseased hive for bacteriological examination. Melissococcus pluton and two species of bacteria would be associated with this disease as secondly invaders were isolated from these dead larvae. The salient characters of isolated M. pluton were as follows: No growth in air and anaerobic growth. No anaerobic growth without carbon dioxide. Requires a ratio of Na: K<1.
These salientj characters, also other cultural characters, were for the most part in agreement with those mentioned in M. pluton by BAILEY.

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We attempted identification and classification of serotypes of acid-fast bacilli isolated from disseminated atypical mycobacterial disease in swine at an abattoir by analysis of mycolic acid and glycopeptidolipid on thin layer chromatography.
All isolated strains were Mycobacterium intracellulare, and strains isolated from nine swine of serialoutbreak at a farm belonged to serotype 8, while the other strains belonged to serotypes 4 and 8.

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Two consecutive abortions of a sow occurred on a swine farm in Hiroshima Prefecture, in October, 1986 and February, 1987. The sow was born in May, 1985 on the same farm. Fetuses and their placentae from the first and second abortions, as well as the sow, were pathologically examined.
At necropsy, no remarkable changes were found in the fetuses collected at the first abortion except for postmortem autolysis. One fetus from the second abortion showed serious autolysis, whereas the other fetus revealed small, whitish foci in the liver and placentae. In the sow, the uterine mucous membrane was brownish in color and the vaginal mucous membrane was covered with a small amount of yellowish mucus.
On histological examinations, a number of small granulomatous foci were recognized in the liver, spleen, king, lymph nodes, cerebrum, skeletal muscles and skin of the fetuses and in their placentae. Similar lesions were observed in the genital organs, liver and intraperitoneal lymph nodes of the sow. The Ziehl-Neelsen staining revealed numerous acid-fast bacilli in those granulomas.
These results indicate that the abortions may have been caused by invasion and proliferation of acid-fast bacilli in the fetuses and their placentae.

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A case of tracheal collapse (TC) within the chest, which was diagnosed by radiograph, was observed clinically in a three years and ten months old male chihuahua dog. This case showed successful reaction to treatment using corticosteroid, bronchodilator and antibiotics during about 28 months in which a period of 12 months consisted of no treatment. At the age of 6 years and 3 months, severe symptoms of TC with respiratory distress with goose honk cough and the protruded tracheal cartilage in the lumen of trachea were observed., The dyspnea was temporarily improved by using a tracheal tube as an emergency treatment, but the patient died on the 4th day after the outbreak of TC. As the pathological changes in the trachea, there were evidence of flattening, stenosis of the trachea, deformation, and protrusion into the lumen of cartilages, hypocellular change and randomly distributed connective tissue fibers in the organic matrix of cartilage, and thickened and flaccid dorsal tracheal membranes. It was considered that this case was a severe grade type, more than grade IV in the classification of TC by Tangner.

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The technique for long term maintenance of central venous catheter is indispensable to total parenteral nutrition. When dogs are kept by this nutrition, there are six important points as follows: 1, material of catheter; 2, insertion site and fixation of catheter; 3, dog jacket; 4, protective coil of catheter; 5, swivel; and 6, injection pump.

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