Total intravenous anesthesia (TIVA) using 1% guaifenesin-0.2% ketamine-0.005% xylazine (GKX) was applied to 9 clinically healthy cows which were premedicated with 0.02mg/kg IV atropine followed by 0.05 mg/kg IV and 0.05mg/kg IM xylazine. Anesthesia was induced by 0.5mg/kg IV ketamine and maintained for 60min by continous intravenous injection of GKX in 6 cases after recumbent, while 0.2% ketamine was injected in 3 control cases. At the end of anesthesia, 3 cases anesthetized with GKX were intravenously injected with atipamezole at a dose of 0.25mg/mg of the total xylazine injected, and control cases were antagonized by 1.0mg/kg IV trazoline. The mean total consumption of TIVA solution was 4.6ml/kg/hr in the GKX group while 8.8ml/kg/hr in the control group. During anesthesia, the acceptable heart rate and arterial blood pressure were maintained, although respiratory acidosis and hypoxemia were observed in both groups. The mean standing-up times after anesthesia in the GKX group were 9 and 63min with and without atipamezole, respectively, while 30min in the control group.
Detection of a common antigen of Gram-negative bacteria was attempted with immunohistochemistry on formalin fixed paraffin sections. An anti-lipopolysaccharide (LPS) rabbit serum widely reacted with gramnegative bacterial antigens in the lesions. In addition, the anti-LPS serum was reactive with the bacterial antigens in the cytoplasm of infiltrated macrophages and giant cells in fibrous tissues where the species specific antisera were not reactive.
Bovine, equine, swine and chicken serum samples collected in the years of 1984, 1989 and 1994 in Niigata Prefecture were investigated for antibody to Chlamydia psittaci by indirect immunofluorescence. Of 260 bovine sera 87.2% were positive, showing a geometric mean (GM) titer of 1: 242.1. Positivity rates were significantly higher in older cattle than in youngers, and in Holstein breed than in Japanese Black. Of 90 equine, 100 swine and 100 chicken sera collected in 1994, positivity rates (GM titers) were 38.9%(1: 97.0), 78.0%(1: 266.9) and 4.0%(1: 65.8), respectively. These results suggested wide-spread Chlamydia infection among farm mammals in Niigata Prefecture.
Periparturient plasma calcium (Ca), parathyroid hormone (PTH) and 1, 25-dihydroxyvitamin D [1, 25-(OH)2D] levels were evaluated in 8 cows with milk fever history (historied cases) and 5 cows without the history (controls). The lowest plasma Ca level was recorded around delivery in both historied cases and controls. Decreased levels of plasma Ca were lower in the 5 historied cases than those of the controls, and one of historied cases developed milk fever, in which the decreasing of plasma Ca levels persisted in spite of the immediate elevation of plasma PTH and 1, 25-(OH)2D values. Although this hypocalcemia recovered after the Ca therapy, plasma Ca value was repeated to decrease at 2 days after the clinical onset associated with an additionally greater elevation of plasma 1, 25-(OH)2D levels.
The distribution of IgA-containing cells (IgA-cells) was immunohistochemically evaluated in the mucosal layer of the bovine teats lactating milk containing inflammatory exudates. The significantly increased number of IgA-cells was observed in the mucosa with severe infiltration of lymphocytes.
Three canine cases with mammary adenocarcinoma and its pulmonary metastases, showing severe generalized seizure, were clinically and pathologically examined. One of them suggested to have multiple or diffuse disorder of the brain by neurologic examination, was diagnosed as multiple tumor metastases by contrast computed tomography, which was confirmed by pathologic examination. Other two dogs were diagnosed as single brain metastasis of the tumor. The primary pituitary adenocarcinoma was disclosed in one case.
A newborn head-sized mass arising in the abdominal cavity in a male Shiba aged 10 years were examined histopathologically and electron microscopically. The mass consisted of spindle-shaped fibrobastlike cells and the stroma rich in collagen fibers. The tumor cells were compactly arranged in a herringbone growth pattern and were immunohistochemically positive for both factor XIII and vimentin. Numerous dilatated rough endoplasmic reticula and microfibrils were seen in the cytoplasm of the fibroblastlike cells. Based on these findings, this case was diagnosed as fibrosarcoma.
In 58 cases of Japanese Black cattle slaughtered in Fukuoka Prefecture from April 1992 to May 1994, eosinophilic phlebitis was detected in the liver. The tube-like or twig-like form lesions were seen on the surface of the liver. Histopathology revealed expanded interlobular veins, villous proliferation of endothelium, remarkable eosinophil infiltration, smooth muscle hyperplasia and proliferation of interlobular connective tissue. The occurrence of this lesion was presumed to participate the hereditary influence, because this lesion was seen on Japanese Black cattle characteristically.