Journal of the Japan Veterinary Medical Association

[title in Japanese]

[in Japanese]

2006 Volume 59 Issue 12 Pages 773-774
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.773

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[title in Japanese]

[in Japanese]

2006 Volume 59 Issue 12 Pages 780-786
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.780

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[title in Japanese]

[in Japanese]

2006 Volume 59 Issue 12 Pages 787-790
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.787

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Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolates During 1999 and 2000 in Japan

Ayako MORIOKA, Tetsuo ASAI, Toshio TAKAHASHI

2006 Volume 59 Issue 12 Pages 815-819
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.815

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By investigating thein vitroactivity of 21 antimicrobial agents that are commonly available for veterinary use against 303 Japanese isoletes ofActinobacillus pleuropneumoniaecollected between 1986-1987 (178 strains) and 1999-2000 (125 strains), the authors compared the susceptibility of strains isolated in different years to the drugs examined. The resistant frequencies of the recent isolates were increased in (P<0.01, χ²test): PCG (change of resistance rate: 1.1%→13.6%), AMPC (1.1%→12.0%), ASPC (1.1%→12.0%), OTC (5.6%→44.8%), CP (1.1%→27.2%) and TP (1.7%→30.4%), which are used mainly for the treatment of disease caused byActinobacillus pleuropneumoniae. In addition, among recent isolates, two of 24 strains typed into serotype 1 were resistant to fluoroquinolones, and one of 76 strains of serotype 2 was resistant to macrolides. Most of the recent isolates were highly susceptible to CTF, FF and fluoroquinolones.

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Molecular Epidemiology ofSalmonella TyphimuriumIsolated from Cattle in Fukushima Prefecture

Masaru SUGAWARA, Tadashi CHIBA, Takaaki OGINO, Hitoshi ITO, Yumiko IMA ...

2006 Volume 59 Issue 12 Pages 820-825
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.820

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Strains of Salmonella Typhimurium (ST) isolated from cattle on 14 farms in Fukushima Prefecture between 1992 and 2004 were analyzed using molecular epidemiological techniques. There were six kinds of ST drug resistance patterns. The experimental results suggested the possibility that the DT104 strains showed resistance to ampicillin (A), chloramphenicol (C), streptomycin (S), sulfonamides (Su) and tetracycline (T), to ASSuT, and to nalidixic acid (N) added to ACSSuT. One amino acid substitution was identified in GyrA of the N-resistant strain. The ACSSuT kanamycin (K) resistant strain was plasmid (170kbp) mediated drug resistant. The ASSuTK-resistant strain possessed a plasmid (120kbp) closely related to an ST pathogenic plasmid, and the PFGE type also differed greatly from that of the other strains. As 13 or 14 strains exhibited a resistance to four or more drugs, it is necessary, from the point of view of both animal health and public health, to monitor the acquisition of new drug resistance in ST.

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Proceedings of the Slide-Seminar held by the Livestock Sanitation Study Grout in 2004 Part I

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

2006 Volume 59 Issue 12 Pages 826-833
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.826

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A Case ofNeospora caninumInfection in an Adult Dog

Toshifumi OYAMADA, Kyoko MITSUMOTO, Hiromi IKADAI, Chun-Ho PARK, Takas ...

2006 Volume 59 Issue 12 Pages 837-842
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.837

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A seven-year-old, male golden retriever suddenly developed bilateral hind limb paralysis. The paralytic condition progressed for almost three months until the dog ultimately died. Pathologically, multiple foci of chronic non-purulent meningoencephalomyelitis with protozoan cysts and/or pseudocysts were widely formed in the CNS, especially in the cerebellum and brainstem. Non-purulent myositis with cysts was also formed in some muscles. The parasites in the cysts were clearly positive forN. caninumimmunohistochemically, and characteristic ultrastructures ofN. caninumwere observed by electron microscopy. The specific band that relates toN. caninumwas detected by nested PCR. The base composition of the DNA products resulted in 98.2% homology with that of N. caninum. From these results, the dog was confirmed to be the first case ofN. caninuminfection seen in an adult dog in Japan.

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Possible Markers Showing the Hygienic Quality of Bulk Milk and Applicability in Estimating Bulk Tank Somatic Cell Counts

Megumi ISHII, Takuei HIRAMA, Hidetoshi HIGUCHI, Hajime NAGAHATA

2006 Volume 59 Issue 12 Pages 847-851
Published: December 20, 2006
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.12935/jvma1951.59.847

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To evaluate useful markers for estimating somatic cell counts (SCC) in bulk milk, electronic conductivity (EC), N-acetyl-β-D-glucosaminidase (NAGase) activity, lactate dehydrogenase (LDH) activity, chemiluminescence (CL) units, adenosine triphosphate (ATP) levels and lactoferrin (Lf) concentrations in milk were measured for comparison with their SCC values. The SCC values correlated significantly (P<0.05, 0.01) with both the CL and LDH values. The measurement of ATP in milk appears to have application in estimating SCC levels in bulk milk.

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