Eighty-four diseased and defective chickens before shipment for meat inspection were obtained from broiler farms and examined pathologically and bacteriologically. Most affected birds showed depression, stunted growth, lameness and paralysis. At autopsy, more than half of all chicken had spondylopathy (59/84 cases),and spinal (corpus vertebrae) abscesses were seen in the affected area in nearly one half of them (27/59 cases). Histopathologically, granulomatous inflammation with gram positive cocci and gram negative bacilli was observed in the vertebral abscess. Salmonella spp., Escherichia coli, and Staphylococcus spp. were detected by the abscess in affected corpus vertebrae and other infectious lesions in a bacteriological inspection. Staphylococcus aureus was detected from only one sample from group C.
A farm that did not have porcine reproductive and respiratory syndrome (PRRS) virus had several pigs found to be antibody-positive in an ELISA (seropositive pigs). The positive rate was 5.4% among growing pigs and 0% among breeding sows and fattening pigs. Diagnostic tests on the five positive pigs ruled out PRRS. Serum of the seropositive pigs showed an ELISA S/P of 0.431 to 3.346, but had an IFA titer of less than 1 : 20, and were negative for nested PCR. It was concluded that all of the seropositive pigs had shown a nonspecific reaction. Investigating the possibility of infection by checking clinical symptoms, and conducting IFA and nested PCR with seropositive pigs appeared to be an effective procedure for verifying nonspecific reactions in an ELISA.
A 16-month-old Japanese Black heifer persistently infected with bovine viral diarrhea (BVD) virus developed emaciation, hyperglycemia, glycosuria and ketonuria. The plasma glucose tolerance and insulin concentration declined markedly to 0.25 of glucose disappearance rate and 2.7 to 4.9 μU/ml, respectively. The major pathological changes were characterized by a decrease in the size and number of pancreatic islets. Residual islets contained islet cells with vacuolated cytoplasm, including small amounts of aldehyde fuchsin-positive granules or insulin antigens. Based on the results, the disease was diagnosed as type 1 diabetes mellitus.
A one-year-old thoroughbred that, immediately following long-distance shipping, had contracted a fever and subsequent renal failure was admitted to our Veterinary Teaching Hospital 21 days after having been shipped. In addition to important clinical laboratory findings such as congested visible mucous membrane, leukocytosis, and azotemia, a gene fragment specific to leptospira interrogans sensu lato was evidently amplified by the culture media from urine deposit and blood. Although the white cell blood count and blood urea nitrogen were normalized around the 11th and 21st days following fluid therapy (75ml/kg/day) and antibiotics administration (cefazolin sodium 50mg/kg/day), polyuria and polydipsia were persistent. Hypercalcemia and hypophosphatemia and an increased ratio of BUN to creatinine (＞10) also developed, and consequently the horse was euthanized on the 33rd day of hospitalization. The necropsy confirmed enlargement of both kidneys and the hyperplasia of the cortex. A case report of equine leptospirosis associated with renal failure suggests the need for a longitudinal epidemiological survey of leptospira interrogans serovars in Japanese horses.
An eight-year-old, intact male toy poodle was diagnosed with diabetes mellitus, and administered with insulin. Hypoglycemia with unknown causes was observed three months prior to the diagnosis. After three years of insulin therapy, insulin administration was discontinued because of intermittent hypoglycemia. Two weeks later, hypoglycemia and titanic seizure occurred, although no insulin had been given. Because glucose infusion or anticonvulsant therapy failed to control the seizure, euthanasia was performed. Necropsy showed diffuse enlargement of the pancreas and seven ashen nodular lesions in the liver. Insulinoma and its hepatic metastasis were diagnosed by histopathological examination.
Three dogs were diagnosed with reproductive tumors after castration or ovariohysterectomy. Since the lesions metastasized or were at risk for metastasis, the dogs were referred for postoperative chemotherapy. Their histopathological diagnoses were ovarian adenocarcinoma disseminating to the peritoneum, ovarian granulosa cell tumor with vascular invasion, and seminoma metastasizing to the celiac lymph nodes. The dogs were treated four or five times with a dose of cisplatin 40-50 mg/m2 intraperitoneally-injected every three weeks. Two dogs showed no signs of metastases for 31 and 7 months, respectively. In the dog with nodal metastasis, administration of intraperitoneal cisplatin five times reduced the hypogastric lymph nodes from 56.4 mm ×26.5 mm to 45.7 mm ×17.9 mm.