Ten dogs were found to be affected with the swelling and extraordinary exposure of the nictitating membrane, or the third eyelid. Clinically, they were diagnosed as cases of abnormal dilatation of glandular cells of the glandula palpebrae tertiae superficialis accompanied by slight, partial hyperplastic change of the gland. Histologically examined, the abnormal glandular dilatation was presumed to have been induced by such intrinsic factor as excessive secretion of the gland itself. The role of extrinsic factor, if any, seemed to be minor or insignificant. Further histopathological studies must be done to determine whether this malady will develop into adenitis or tumor.
Toxoplasma (Tp) organisms and cysts were examined for resistance to high and low temperatures. The infected materials used were the liver and muscle of infected hogs collected from the Shibaura, Tokyo, Slaughterhouse, ascites of mice inoculated with Tp, and the brain harboring cysts of mice inoculated with the Beverley strain of Tp. They were kept at a certain temperature for a given time and then made to emulsion. The resulting emulsions were inoculated into mice and underwent several passages through mice. After that, ascites was collected from these mice and examined for the presence of Tp organisms. As a result, the infectivity of Tp to mice was lost in natural materials kept at -10°C or below for a day. It was not damaged in the infected mouse brain harboring cysts and kept at-10°C for two days. It was maintained in both naturally and experimentally infected materials treated at 50°C for five minutes. It was destroyed, however, in these materials treated at 55°C for five minutes.
A total of 605 bovine sera were collected from cattle in the Kyushu and Chugoku regions and Jersey cattle which were introduced in Japan from Australia in 1960, at the time of arrival in Japan. They wer tested for antibody neutralizing IBR virus. The positive rate was 7.1 per cent in the Kyushu region. No positive sera were detected from she Chugoku region and among the Jerseys. There were no differences in positive rate between the sexes, ages, and breeds. The antibody titer of positive sera ranged from 1: 2 to 1: 64. IBR virus may be present in Japan.
Inoculation of 40-day-old piglets with 10cc of hog cholera crystal violet inactivated vaccine (CVV) gave rise to a mild, transitional postvaccinal reaction in them, but that with 10cc of hog cholera tissue culture vaccine (TCV) hardly caused such reaction. A dose of 10cc was more than three times the conventional dose for a young pig weighing less than 20kg. Piglets inoculated with 10cc of CVV or TCV at the age of 30 days, or 10 days before weaning, acquired immunity a little stronger than those injected with 3 or 6cc at the same period, but a little weaker than those vaccinated with 10cc at the age of 40 days, or 2 days after weaning. They withstood challenge inoculation with hog cholera virus made 3 months after vaccination, exhibiting mostly mild or severe reaction. Some piglets received a total amount of 9cc of CVV divided into 3 doses of 3cc each at 5 days' intervals beginning with 30 days after birth. Others were inoculated with 2 doses of 4.5 cc each of CVV at 30 and 40 days of age. All of them were weaned 40 days after birth. As a result, they withstood challenge inoculation made 3 months after vaccination, as well as piglets injeted with a single dose of 9cc of CVV 40 days after birth, showing mild or no reaction. Taking the results of two previous experiments of theirs into consideration, the authors concluded that even piglets and young pigs would be expected to acquire such immunity as equal or close to that of adolescent or adult pigs, if an adequate dose of vaccine was inoculated at an appropriate time (age and before or after weaning) or at suitable intervals of time.