From February to March 1990, a farmer breeding about 120 milk and hybrid cows administered vitamin A, D, E preparations (A: 2, 000, 000IU/day, D: 1, 000, 000IU/day, E: 800IU/day) everyday to 15calves ranging in age from 7 to 10days. Consequently, all animals showed a loss of vigor, anorexia, depilation, and dysbasia, and 6calves died 13-27days after the start of the administration. Three were autopsied and found to have brownish-red depositions on the endothelial surface of the aorta and pulmonary arteries and slight discoloration of the liver. Histologically, swelling and vacuolation were noted in the hepatic Itoh cells, The blood chemical test detected serum retinal palmitate in 6 of 8cases (75.0%). All animals showed remarkable increases in plasma 25-OH-D (561.9±77.4ng/d
l) and serum Ca (14.4±1.3mg/d
l). The above findings led to a diagnosis of these cases as hypervitaminosis AD, and a pre-hyena disease state was suspected in view of physical occlusive insufficiency.
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