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Volume 42, Issue 11
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HIROSHI KODAMA1989 Volume 42 Issue 11 Pages 751-756
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (893K) -
NOBUO TSUNODA, HIROAKI AKITA, KIYOSHI MIYAZAWA, KAZUE OOSAKI, KUNITADA ...1989 Volume 42 Issue 11 Pages 759-762
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA commercial Kit (OVUCHECK Plasma/Serum ELISA Kit, Cambridge Life Sciences, England) designed to measure the concentration of progesterone using an enzyme linked immunosorbent assay (ELISA) has been assessed for measuring progesterone in the serum of mares.
Maximum time required for the entire procedure was 3 hours. The range of progesterone levels measurable by this kit was 0.5 to 10.0 ng/ml serum and correlation between the results by this method and those of RIA was significant (r=0.87, P<0.01). The intra-assay and inter-assay coefficients of variation were 10.9-12.8%(1.6-7.8 ng/ml, n=10) and 8.7-16.5%(0.2-4.4 ng/ml, n=10), respectively.
Changes in the circulating levels of the progesterone can be detected by the Kit; in particular, low concentrations during the follicular phase and the subsequent rise during the luteal phase of the oestrous cycle have been determined.View full abstractDownload PDF (515K) -
SEIICHI KAWAMURA, KIYOSHI SAKAI, NAOKO WATANABE, YOSHIMASA WATANABE, N ...1989 Volume 42 Issue 11 Pages 763-769
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSBromsulphalein half life (BSP-t/2) with fractional clearance (K value) was investigated in cattle by a modification of the method previously reported by the present authors. Mean biological BSP-t/2 value (minutes) of 23 dairy cows (Holstein) and 21 beef cattle (Japanese Shorthorn) was 3.31 min [range of 95% confidence limit (CL): 2.74-3.88 min] and 3.53 min (2.76-4.30) with K value 20.9%/min (range of 95% CL: 17.9-25.3%/min) and 19.6 (16.1-25.1). Each clearance curve plotted in mean BSP concentration of two cattle groups was related to time until 12 min after injection, rectilinearly. The cattle examined were confirmed to reveal no abnormality in the activities of serum enzymes reflecting liver function. plasma concentration of lipids and albumin-globulin ration of serum.
On the other hand, BSP-t/2 values and K values in 5 cows with liver disturbance due to CCl4 intoxication showed progressively changes and exhibited a significantly high correlation between the values of BSP percent retention (BSP-PR), enzyme activities and lipid concentration in serum, and triglyceride (TG) and glycogen (GL) contents in biopsy specimens. Degree of diffused liver lesion (hepatosis) was estimated from biological and morphological findings of each specimen examined, and classified into four types relative to BSP-t/2 values for each type. BSP-t/2 values indicated higher value in, the values before CCl4 administration, inactivated type of chronical hepatosis, activative chronical hepatosis, acute hepatosis and fulminant hepatosis. No overlap within the 95% range CL of any type of hepatosis was recognized.View full abstractDownload PDF (881K) -
NAOYUKI TAKEMURA, HIDEKAZU KOYAMA, TOSHINORI SAKO, MASAHIRO TAGAWA, SH ...1989 Volume 42 Issue 11 Pages 771-773
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe present study was made to investigate the effects of the initial stage of filariais on canine cardiac function. Controls (n=10, BW 7.9±0.4kg, mean±SEM) and dogs with heartworm (n=9, BW 8.8±0.3 kg) were used. Electrocardiographic examination detected that the amplitude (3.8±0.3 mV) and duration (3.9±0.2 msec) of the P wave were increased in dogs with heartworm. Catheteric examination of the heart revealed the cardiac index (5.1±0.21/min/m2) to be significantly decreased (p<0.005), and right and left ventricular end diastolic pressures (7.3±0.7 and 12.6±2.1 mmHg) were greatly increased (P<0.025), compared with the controls. These results might indicate the presence of cardiac dysfunction in the initial stage of filariasis. The detail mechanism in the development of cardiac dysfunction is still unknown.View full abstractDownload PDF (2065K) -
KIYOSHI TOMINAGA, MUNEO NAKAZAWA, MAKOTO HARITANI, KOHICHIRO HIRATA1989 Volume 42 Issue 11 Pages 775-779
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSAtypical strains of Escherichia coli were isolated from two calves with watery diarrhea and/or dysentery in 1986 and 1988. The distinctive biochemical characteristics of the isolates were anaerogenic, non-motile and production of urease; their serotypes were 05: K+: NM. All of an atypical E. coli from two calves produced verotoxin, but were negative for ST and LT enterotoxins. The colostrum-deprived kids inoculated orally with 109 viable bacteria of an isolate of E. coli, 05: K+: NM, excreted loose, mucoid feces at 56hr postinoculation and produced a mild, focal colitis with typical bacterial attachment and effacement lesions. From these results, we considered that this 05: K+: MN strain of E. coli has the ability to produce colitis characterized by attachment of bacteria to the enterocyte surface and effacement of microvilli in the large intestines, as reported by Moon et al. and Hall et al. This is the first report of “attaching and effacing E. coli (AEEC)” as the cause of calf diarrhea in Japan.View full abstractDownload PDF (3525K) -
SHIN SERIKAWA, NAOHITO KUSAKARI, TSUTOMU OHGI, KAZUHIRO SENNA, HIROMI ...1989 Volume 42 Issue 11 Pages 781-785
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn January 1985, an outbreak of listeriosis occurred in 3 flocks of 232 pregnant ewes fed hay and grass silage in Takikawa Animal Husbandry Experiment Station, Hokkaido. In total, twenty-six ewes presented encephalitic signs, six of them died and 10 were culled. Listeria monocytogenes was isolated from 11 brain samples of 15 carcasses. The isolates were serotyped as type 4b and were highly sensitive to many kinds of antibiotics except sulfisoxazole, colistin, polymixin B and nalidixic acid. Fifteen of diseased 26 ewes were treated with oxytetracycline; 10 ewes with mild symptoms recovered. L. monocytogenes was also isolated from 3 of 10 specimens of the same silage, which suggested that the silage contaminated with these organisms was responsible for the outbreak.View full abstractDownload PDF (763K) -
KAZUFUMI UEMATSU, MASAHIRO NODA, SHIRO WATANABE, MASANORI MOUKO, HIROO ...1989 Volume 42 Issue 11 Pages 787-791
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSTwo pregnant sows with watery diarrhoea died suddenly, one after the other, just before delivery on a swine farm in Hiroshima Prefecture in February, 1988. Salmonella (S.) lockleaze belonging to biobar I and 0-7 group of Salmonella was isolated significantly from these carcasses as a causative agent. No virus was isolated from these materials. In the bacteriological examination of rectal swabs of 223 sows from this farm, three carriers of S. lockleaze, including one case with diarrhoea, were revealed, and S. derby and S. londonwere each separately detected in two and S. anatum was detected in other two apparently healthy sows. No prasmids associating with their pathogenicity were detected from S. lockleaze. This is the first report on the isolation of S. lockleaze in Japan.View full abstractDownload PDF (684K) -
SATOSHI FURUKAWA, HIZURU TAKAMI, YOUKO MORI, SUSUMU NOMURA, YOSHIKI KO ...1989 Volume 42 Issue 11 Pages 793-796
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe prevalence and severity of swine enzootic pneumonia (SEP), atrophic rhinitis (AR) and pleuropneumonia were examined in 83 fattening pigs from a farm.
About 92% of the pigs had on SEP lesion, 36% had AR, and 29% had pleuropneumonia. Positive correlations were found between retarded body weight gain and severity of SEP, and between retarded body weight gain and existence of AR lesions.
The pigs with severe AR lesions tended to exhibit widespread SEP lesions.View full abstractDownload PDF (594K) -
TAKEHITO OGATA, ISAO NAGANO, TAKAAKI HASEGAWA, TAKASHI SAHASHI, KATSUY ...1989 Volume 42 Issue 11 Pages 799-802
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSChlamydial complement fixation (CF) antibodies were investigated in 509 cattle and 506 pig sera. Antibody prevalence in Cattle was about 15.9%, and there were some differences in positive rate among cattle producing prefectures in Japan. The positive rate of antibody against C. psittaci of cattle aged over 3 years was higher than that of cattle aged about 2 years. However there was no correlation between gross pathological lesions and positive rate of antibody. On the other hand, antibody prevalence in pigs was about 1.2%, suggesting that porcine chlamydial infection was present in Japan.View full abstractDownload PDF (475K) -
KOHKICHI GOTOH, HIROSHI SATOH, HIROSI WATANABE, MAKOTO IWASAWA, CHIKAK ...1989 Volume 42 Issue 11 Pages 803-806
Published: November 20, 1989
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA malignant mastocytoma of the tongue was encountered in a sow. Greyish-white nodules, varying from 0.5 to 2.0 cm in diameter, fused to form irregularly shaped papulous masses on the dorsum linguae. Tumor nodules were primarily distributed in the submucosal portion and invaded surrounding tissues. Larger nodules located at the periphery of the tumor mass had necrotic areas. Neoplastic mast cells had round or ovoid nuclei with slight atypia, hyperchromasia and a few mitotic figures. Polyhedral broad cytoplasm contained metachromatic granules visualized by toluidin blue stain. Electron microscopy revealed that mast cell granules of this tumor were composed of fine grains. Microscopic metastatic foci were clearly detected in the submandibular lymph nodes. In the liver, there were small mast cell foci among uneven interlobular connective tissue septa as seen in typical cases of so-called fibrotic hepatosplenomegaly or systemic mastocytosis of pigs. These findings appear to be suggestive for revealing the true nature and correlation among mast cell disorders in this species.View full abstractDownload PDF (7407K)
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