The Takata and Gros tests, related to some extent with changes in the serum proteins, were carried out on the serum of infected dogs and control. The Gros test was very sensitive and gave positive results when applied to cases exhibiting an increase in the globulin fractions and a decrease in the albumin fraction. The Takata test revealed a strongly positive reaction when applied to patients having an accumulation of ascites and showing a surprising rise in gamma-globulin, but usually gave a negative reaction when applied to carriers and non-infected dogs. Some of the patients having an accumulation of ascites showed a tendency that total cholesterol level decreased whereas others retained a high level of this substance. When treated with sodium antimony tartrate, infected dogs manifested a temporary decrease in gamma-globulin and a corresponding increase in alpha-2 globulin soon after the first dose was injected. Ninety days after injection, there was a tendency for gamma-globulin and total protein concentration to increase. Following the administration of carbon tetrachloride, alpha-2 globulin showed a temporary rise, albumin a consistent decrease, and gamma-globulin a progressive increase. In dogs which died of tetrachloroethylene poisoning, the most remarkable change of the serum protein fractions was an increase in alpha-2 globulin. Therefore, it was observed that serum protein changes in an individual case of canine filariasis indicated the reaction of the patient to the liver dysfunction. These changes appeared in a comparatively early stage of the impairment. The elucidation of these changes added something to the information on the injuriousness and effect of anthelmintic treatment on the host of parasite.
First trials were conducted in the Sanjiro Paddocks of the Utsukushigahara Pasture in Nagano Prefecture during the summer of 1961, so that it might be determined whether free-living ticks on the pasture could be controlled effectively with pesticides applied by the helicopter. Three formulae of 1.5% diazinon dust and one formula of 3% BHC dust were applied to 3 experimental plots of about 100 hectares each at a dose of 0.3 kg per hectare. Investigation was carried out on the following items: suitable flying conditions of the helicopter for dusting and dispersion, deposit and tickicidal effect of the dusts and their influence upon livestock. The results showed a considerable drop in the tick population on the ground and no undesirable side effect of the dust upon cattle and sheep in the paddocks.