The anthelmintic efficacy of thiabendazole was evaluated in field tests on 34 cattle and critical tests on 8 chickens. A dose of 50mg of thiabendazole per kg of body weight, given in a drench or a ration, was not sufficient to remove parasites of the genera Haemonchus, Mecistocirrus Ostertagia, Cooperia, and Nematodirrus from cattle. A dose of 100mg/kg, given in a drench, showed a considerable efficacy against these parasites. Both doses had little effect upon worms of Trichuris and Moniezia. The drug was proved to be safe for use in cattle. When administered orally to chickens, 100mg/kg of thiabendazole had a low efficacy and 300mg/kg an uncertain efficacy in removing Ascaridia galli, but 500mg/kg showed complete effectiveness against this species. These three doses had little effect on Heterakis gallinae. The drug might not be promising because of its relatively high dose rate, though it was proved to be safe for use in chickens.
A total of 1, 459 pigs received by 14 slaughterhouses in Saitama Prefecture were subjected to serological tests of DT, HA, and CF to clarify the distribution of toxoplasma (Tp) antibody all over the prefecture. The positive rates obtained were 21.7 per cent in DT (≥16×), 22.6 per cent in HA (≥20×), and 16.8 per cent in CF (≥4×). DT and HA were both positive in 242 head (16.6 per cent). The Tp-positive rate among slaughtered pigs was 16.2 per cent throughout the prefecture, and 14.5 and 18.7 per cent in the northern and the southern half of the prefecture, respectively. It was 54.9 per cent in the Shirako Slaughterhouse, being significantly high as compared with the rate in any other one. Of the nine counties of the prefecture, Kita-adachi showed the highest positive rate, being 28.1 per cent, among the pigs produced and sent to slaughterhouses, and Minarnisaitama the lowest positive rate, being 7.7 per cent. When a total of 165 employees were examined in the 14 slaughterhouses, the positive rates were 58.2, 41.2, and 27.3 per cent in DT, HA, and CF, respectively. Both DT and HA were positive in 64 persons (38.8 per cent). Among 18 meat inspectors, the positive rates were 33.4, 22.2 and 16.7 per cent in DT, HA and CF, respectively. Three inspectors (16.7 per cent) gave positive tests of both DT and HA. All positive reactors had been working at slaughterhouses in the southern half of the prefecture.
Ultraviolet ray-inactivated rabies vaccine was examined for ability of producing neutralizing antibody and capacity of protecting inoculated animals from infection due to later challenge with virus. Goats were used for these purposes. As a result, this vaccine was proved not to be inferior to the vaccine derived from the Flury strain in these regards.
1. P-MeC (containing 10mg/kg of MeC) was injected intramuscularly into two suckling piglets. The blood levels (unit:μg per ml of serum) determined in the two animals at certain intervals of time after injection were 28.0 and 30.6 at 1 hour, 19.0 and 16.0 at 4 hours, 14.5 and 16.0 at 6 hours, and 2.35 and 2.85 at 24 hours. 2. In normal healthy piglets, the average value of Escherichia coliwas 9.20 at 1 week of age, 8.84 at 2 weeks, 8.04 at 3 weeks, 8. 15 at 4 weeks, 7.31 at 5 weeks, and 8.60 at 6 weeks. In piglets suffering from diarrhea, it was in a range of 6.78 to 12.18, averaging 10.30. 3. The growth of E. coli organisms derived from the feces of the piglets affected with diarrhea was inhibited to a considerable extent in SLSHI medium to which P-MeC and MeC-sensitive disks had been added. 4. A total of 105 cases of diarrhea in suckling piglets were effectively treated with P-MeC (containing about 1.26 to 7.08mg/kg of MeC) administered by the intramuscular route. 5. No side-effect was observed in any piglet treated.