A practical polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify bovine papillomavirus (BPV) genotypes was developed and was compared to the conventional method (CM). Bovine papillomas were collected from dairy heifers in a grazing facility in Shizuoka. The CM detected BPV9 (19/92), BPV6 (56/92) and BPV10 (7/92) and all positive samples could be identified by PCR-RFLP. These results suggested that PCR-RFLP is a useful method for identifying BPV genotypes.
A 14-year-old, neutered female mixed-breed cat was presented for vaccination. Physical examination revealed the cat had a mass in the ventral cervical region. Blood testing revealed hypercalcemia, increased ionized calcium and intact PTH levels. The mass was removed surgically. Diagnosis of parathyroid adenoma was based on histopathological findings. After surgical treatment, an immediate decrease was observed in all items at an abnormal level. The total calcium level reached the reference range, but ionized calcium and intact PTH levels were slightly outside the range. The cat was diagnosed with chronic renal failure approximately seven months later and treatment is still being provided.
We retrospectively evaluated 977 dogs immobilized with intravenous medetomidine injection for examination or treatment at the Gifu University Veterinary Medical Center to clarify the incidence of ventricular premature contraction (VPC). Of these, 43 (4.4％) had VPC confirmed by electrocardiography. Using age, body weight, gender, breed, and presence of heart murmur as outcome measures, we performed multivariate statistical analyses to identify the risk factors for VPC during immobilization. Our multivariate analysis showed that two breeds, Labrador Retriever and Golden Retriever, had significant risk factors for VPC.
Effects of a single disinfectant of didecyldimethylammonium chloride (DDAC) (single A) and a compound disinfectant (compound B) consisting of DDAC and phenolic compounds, with limewater or distilled water on avian influenza virus (AIV) and bovine enterovirus (BEV) were investigated at low temperature and with organic matter contamination. Both disinfectants showed a virucidal effect toward AIV. Using limewater increased these effects, but compound B showed higher effects than single A at low temperature and with organic matter contamination. Single A and compound B were effective against BEV with limewater, but not in the distilled water. These effects were significantly reduced at low temperature and with organic matter contamination, but compound B displayed virucidal effects against BEV. It was concluded that single A and compound B with limewater was more effective against AIV and BEV than with distilled water. And compound B showed more virucidal effects at low temperature and with organic matter contamination.
The prevalence of Sarcocystis cyst (sarcocyst) in myocardium and four cuts of carcass (chuck, rib roast, flank and round) from 30 heads of discarded dairy cattle (Holstein breed) was investigated. Sarcocysts were detected from all myocardium samples (100%) - 13 chuck (43.3%), 10 roast (33.3%), 10 flank (33.3%) and 11 round samples (36.7%). The numbers of cysts in four cuts of carcass were lower than those in the myocardium. The average number of sarcocysts detected per tissue section was 8.7 (range 1-58) for myocardium, 2.0 (1-5) for chuck, 1.1 (1-2) for rib roast, 1.9 (1-5) for flank and 1.8 (1-5) for round. We also investigated for sarcocyst contamination in beef for retail purposes in a meatpacking company. Fifty-six beef specimens were derived from 26 crossbred beef cattle and 30 Japanese black beef cattle. No sarcocysts were detected in the former, but they were found in five samples of the latter (16.7%). One, one, three, eight and 11 cysts were detected in the respective specimens. Morphological examination identified these sarcocysts as Sarcocystis cruzi.