Bromosalicylanilide (proprietary name: Kaken D-4194) is a derivative of salicylanilide and has an anthelmintic effect on liver flukes. It was first examined for toxicity for rabbits and goats, and then for destructive effect upon naturally infected cattle. 1) A dose of 100mg/kg killed 2 rabbits 15 hours after medication. A dose of 200mg/kg killed a rabbit in 14 days, but was not lethal to another one. The fatal animals showed dyspnea and notable congestive pulmonary edema. 2) A dose of 50 or 100mg/kg was not lethal to goats. A dose of 200mg/kg killed a goat in 39 hours. The dead case revealed dyspnea and cardiac dysfunction. All the animals, killed and survived, had congestive pulmonary edema. 3) A total of 26 cattle naturally infected with liver flukes were given an oral dose of 25mg/kg. Egg examinations conducted on the 11 th-12th the 23rd-24th, and the 36th-37th day gave a negative conversion rate of 88.5, 96.2, and 96.2 per cent, respectively, as compared with the results before treatment. In appetence a decrease in milk yield, and soft feces were seen in some animals only for 1-2 days. Physical, cytological, and chemical tests were carried out in parallel. From the results of these tests, it was concluded that a dose of 25mg/kg of this drug had an excellent anthelmintic effect upon liver flukes.
In search of the enteroviruses in cattle from Kumamoto, nine isolates cytopathic for bovine kidney cells were recovered from fecal specimens. Judging from their cytopathic effect, small size, resistance to ether, RNA type, and other properties, they were presumed to belong to the ECBO virus group. Serologically, they were classified into three distinct types, which were tentatively designated as Types 1, 2, and 3. Type 1 was closely related to BF1 described by Inaba et al., but the others were not. The isolates were passed consecutively in various cell lines, such as BK, chick-embryo fibroblasts, HeLa, Ps, and TC cells yielding cytopathic changes. The progeny began to appear in 6-24 hours. The infective titer reached its maximum in 24-96 hours after inoculation. Hemagglutinin was produced parallel with the growth curve. It is interesting to note that hemagglutinin production varied according to strain, type of cell, and passage. A total of 709 serum samples from cattle, horses, swine, goats, dogs, and human beings were then tested for neutralizing antibodies.All of them, except those from dogs, had the antibodies against Types 1, 2, and 3 with thefollowing frequencies: 56.0, 17.7, and 42.5 for cattle, 37.5, 6.3, and 0 for horses, 59.7, 70.3, and 30.0 for swine, 25.5, 22.5, and 0 for goats, and 78.5, 94.3, and 0 for human beings, respectively. This finding strongly suggests that the infections of the three type ofviruses are widely spread not only among cattle but other species of animals. and human beings.