Diethylcarbamazine (proprietary name: supatonin) was tested for anthelmintic effect on Metastrongylus by administering it to repeatedly infected experimental cases in the early stage of infection. The following results were obtained. 1. In the first experiment, treatment was started 14days (in groups A, B and C), 18days (in group D), and 21days (in group E) after the first infection with metacercariae of Metastrongylus. The deworming rate was 93 per cent in group A (treated with 5 courses at intervals of 13 days, each course consisting of 3 daily doses of 100mg/kg each given every other day), 88 per cent in group B (treated in the same manner as group A, except the daily dose which was 150mg/kg), 91 per cent in group C (treated in the same manner as group A, except that each course consisted of 5 daily doses of 100mg/kg each given for as many consecutive days), 98 per cent in group D (treated with 4 courses at intervals of 17 days, each course being the same as in group C), and 93 per cent in group E (treated in the same manner as in group D, except an interval between courses which was 20days). It was 93 per cent on the average in the 5 groups. 2. A second experiment was carried out to co nfirm the most excellent results in the preceding experiment that had been given by group D. As a result, it revealed a similar deworming rate of 94.5 per cent. 3. No anthelmintic effect of the drug was proved in group F in which treatment was started 25 days after infection with metacercariae when the worms began to ovulate.
The actual state of occurrence of so-called slimy condition was investigated in Vienna sausage. More over, some conditions required for the occurrence and the cause of slimy condition were examined. The results obtained are summarized as follows. 1. When allowed to stand under appropriate conditions, commercial Vienna sausage gave rise to slimy condition without exception. influenced by temperature and the moisture of the superficial portion of a given product of sausage. So long as the temperature was 4, 20, or 37°C, the higher were the temperature and the superficial moisture, the more rapidly slimy condition occurred. 3. It was concluded that slimy condition was brought about by the multiplication of bacteria in the superficial portion of sausage and that a principal component- of the slimy substance produced was an aggregation of bacteria.
A disease broke out among ducklings in Ibaraki and Saitama Prefectures from February to August in 1963. It brought about a fatality rate of about 50 per cent. A virus was isolated and proved serologically to be identical with the virus of infectious anatine hepatitis which broke out in Chiba Prefecture in 1962. It was strongly pathogenic only for young ducklings. Neutralizing antibody was not observed in the serum of ducklings which were prior to clinical infection, but was present in that of surviving ones (35 to 50 days old) in a titer of more than 2.0. Then experiments were carried out to determine whether the serum of such surviving ducklings was effective to prevent infectious anatine hepatitis or not. The results obtained are as follows. 1. Three-day-old ducklings were injected intramuscularly with 0.1, 0.5, or 1.0cc of serum. When they were inoculated with concentrated virus 4 hours after serum injection, they survived infection without exception. 2. In some area where the infection was prevailing, 3-to 8-day-old ducklings were injected with not less than 0.3cc of serum. As a result, no death occurred to any one of them. In conclusion, it seems that 3-to 8-day-old ducklings in epizootic areas may be prevented effectively from this infectious disease when they are injected intramuscularly, before the establishment of infection, with 0.3 to 0.5cc of serum of ducklings surviving infection with antibody of high titer.