Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 70 , Issue 8
Showing 1-4 articles out of 4 articles from the selected issue
Farm Animal Medicine and Animal Health
  • Shinichi SAKAKIBARA, Hiroshi KANNO, Satoshi TACHIBANA
    Type: Original Article
    2017 Volume 70 Issue 8 Pages 511-515
    Published: August 20, 2017
    Released: September 20, 2017
    JOURNALS FREE ACCESS

    This study evaluated the efficacy of the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in environmental samples as a screening test to determine a herd's paratuberculosis (PTB) status. MAP DNA was detected in environmental samples from 29 herds at the point of detection of MAP-infected cattle. A logistic regression model was used to assess the association between the probability of detection and the amount of viable MAP in the feces (shedding level: SL) of MAP-infected cattle. The probability of detection increased with increasing the SL of MAP-infected cattle. The SL of MAP-infected cattle associated with a 90% probability of detection was 7.8×101 CFU/g. Environmental sampling will make it possible to detect herds including less infectious cattle, and it is simple and cost-effective. It is suggested that repeated environmental sampling will contribute to the progress of the PTB eradication program in Japan.

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  • Takumi CHIKUBA, Mariko TSUJI, Akihisa ITOU, Tomohiko SHIMODA, Ryouta S ...
    Type: Short Communication
    2017 Volume 70 Issue 8 Pages 516-521
    Published: August 20, 2017
    Released: September 20, 2017
    JOURNALS FREE ACCESS

    Neonatal piglets less than one week old had watery yellowish feces on a swine farm in Mie Prefecture, Japan in August 2014. Although the diarrhea ended in the farrowing house after eight days, fifteen piglets died in the meantime. At the necropsy, common gross lesions included mesocolonic edema. The colonic contents were assayed with a commercially available enzyme immunoassay that detects Clostridium difficile (CD) or its toxin, and CD was detected in piglets that had fibrinous colitis. CD was isolated from the colonic and/or rectum contents of the piglets. Histopathologically, segmental erosions were observed in the colonic mucosa, producing volcano lesions (exudation of neutrophils and fibrin into the lumen). Gram-positive bacteria and Clostridium antigens were detected around segmental erosion by Gram staining and immunohistochemical staining using anti-Clostridium. The present case was suspected to be Clostridium difficile infection.

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Small Animal Medicine
  • Makoto TSUJI, Koki UEMOTO, Tetsuya KAWASAKI, Yasuo ISHIZUKA, Takeshi I ...
    Type: Short Communication
    2017 Volume 70 Issue 8 Pages 523-528
    Published: August 20, 2017
    Released: September 20, 2017
    JOURNALS FREE ACCESS

    A 5-year-old spayed female Abyssinian cat had intermittent vomitting. Using gastrointestinal endoscopy, a massive lesion was found in the gastric mucosa. A diagnosis of Helicobacter infection was made, and gastric small B-cell lymphoma was diagnosed using cytopathology and histopathology of endoscopic samples. However, the samples were negative for lymphoid clonality. The cat was treated with Helicobacter eradication therapy for 30 days without any anticancer drugs. As a result, the symptoms resolved, and the lymphoma achieved complete remission. The recurrence of lymphoma in the gastric mucosa was not observed after one year of therapy, although a mild Helicobacter infection presisted.

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Veterinary Public Health, Wildlife and Environmental Conservation
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