In bitches, pregnancy was first detected by a real-time ultrasound scanner on Day 21 after artificial insemination, by palpation of the abdomen on Day 25 and by roentgenography on Day 45. By using the scanner, it was possible to observe details of the fetal body clearly with the advance in pregnancy. The fetal mass in the uterine cavity was first demonstrated on Day 21. The fetal heart beat could be detected on Day 30, the bone system on Day 35, the stomach on Day 40, the urinary bladder on Day 45, and the pelvis on Day 55.
Activities of serum enzymes were examined in 60 calves (41 Japanese Black and 19 Aberdeen Angus) 10 to 100 days of age kept with calves suffering from leukomyoma in the same barns.A marked increase in GOT, GPT, CPK, and LDH was observed in many calves. A very low level of serum tocopherol was found in some calves. These findings made it clear that there weremany calves with a subclinical type of muscle disturbance. In these calves, medication with a-tocopherol was so effective that the serum enzyme activities returned to a normal range within 2 weeks after injection. It showed a further preventive effect. The dams of these calves were found to have been fed a ration containing markedly concentrations of tocopherol and selenium.
Ten Holstein-Friesian streers 12-16 months old were implanted subcutaneously at the base of the ear with 20 mg of estradiol benzoate and 200 mg progesterone. They were slaughtered 1, 2, 3, 4 and 5 months after implantation, two at a time. The concentrations of these steroids in blood and tissues were measured by enzyme immunoassay. They serum level of estradiol-17β increased, reaching a peak 2 months after implantation, and decreased thereafter to the basal level. No significant changes were obscrved in progesterolle levels. The concentrations of estradiol-17βin liver, kidney and muscle increased significantly 2-4 months after implantation, but not in any other tissue. The tissue concentrations of progesterone did not increase significantly. The residual levels of estradiol-17 β and progesterone in all the tissues and blood were lower than the physiological levels in cows during estrus or the late stage of pregnancy. The recommended doses of estradiol benzoate and progesterone implanted caused no harmful residual levels of these steroids in bloodor tissues for human consumption
A new detection method of bovine Sarcocystis cysts (sarcocysts) was devised. In it, a small piece (about 2×5×0.5cm in size) of cardiac muscle was cut out at rightangles to the fibers and examined by a binocular dissecting microscope with illumination from above. Sarcocysts could be observed as minute whitish bodies. The new method was superior to the conventional one in the following points: Fresh sarcocysts could easily be removed from muscle with a dissecting needle. They could be used for morphological examinationin order to identify the species and for experimental infection of final hosts, such as dogs. The new method was applied to 104 healthy cattle slaughtered. It revealed that the incidence rate of sarcocysts increased with the advance in age, that it was 100% among cattle more than 5 years old, and that the larger the cyst, the larger the number of bradyzoites contained.
Dermatophytosis Caused by Microsporum gypseum was found in 2 male and 1 female puppies born from a mongrel 6 years old with brown hair. Primary lesions were observed simultaneously at about 2 weeks of age. By 1 month of age lesions were scattered on legs and tail, and small lesions on back and abdomen. Microsporum gypseum was purely isolated from hair and scale. The dam of the puppies had no lesions or dermatophytes detected. Thepuppies were treated by oral administration with 22 mg/kg of griseofulvin and topical application with an iodine preparation (Isodin). The lesions were completely cured in about 1 month. M. gypseum was isolated abundantly. From the soil of the birth place.
A 55-day-old pig developed edema on the forehead. Histopathologically, accumulation of round cells was seen in the nasal mucosa. The epithelial cells of the tubuloalveolar glands were enlarged to contain large basophilic intranuclear inclusion bodies positive for Feulgen's test. Electron microscopically, a large number of immature virus particles were demonstrated in nuclei, and mature virus particles in cytoplasmic vacuoles in the epithelial cells of the tubuloalveolar glands.