Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
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Volume 24, Issue 3
Displaying 1-7 of 7 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 24 Issue 3 Pages 113-120
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.113
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    1971 Volume 24 Issue 3 Pages 120-121
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.120
    JOURNAL FREE ACCESS
    Download PDF (1968K)
  • Tissue Culture and Attenuation of Avian Infectious Bronchitis Virus
    I. Tissue Culture and Passage of Virus
    S. YAMADA, M. OCHIAI, H. FUJIKAWA, Y. UCHIFU, Y. KODA
    1971 Volume 24 Issue 3 Pages 121-125
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.121
    JOURNAL FREE ACCESS
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    Infectious bronchitis virus was propagated in chicken kidney cell culture, showing cytopathogenic effect (CPE). It also multiplied in chick-embryo cell culture, but failed to exhibit CPE. Neither virus propagation nor CPE was observed in cell cultures of first generation from the kidney of cattle, swine, goat, dog, rabbit, guinea pig, or hamster, or from the mouse embryo, or in passage cells of BHK-21 (hamster kidney origin), PK-15 or PS (swine kidney origin), or Vero (green-monkey kidney origin).
    There were no differences in the appearance of CPE on culture cells, resistance to heating at 37 or 56°C, or antigenicity after virus inactivation between a strain obtained from passage in chicken kidney cells and the initial strain. The former was distinguished from the latter, since it was well propagated in chicken kidney cells, delayed in multiplying in embryonated hen's eggs, and weakly pathogenic for chickens and chick embryos.
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  • Tissue Culture and Attenuation of Avian Infectious Bronchitis Virus
    II. Safety and Immunogenicity of Attenuated Virus
    Shinji YAMADA, H. FUJIKAWA, Y. UCHIFU, Y. KODA
    1971 Volume 24 Issue 3 Pages 126-129
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.126
    JOURNAL FREE ACCESS
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    The Nerima strain is infectious bronchitis virus attenuated by passage in chicken kidney cells. It was proved to be high in safety, since it produced no reaction to 3-day-old chicks showing an antibody titer of 0.5≥or 30-to 70-day-old chicks with no antibody.
    The amount of virus required for immunization was 104.0 TCID50≤per capita. Neutralizing antibody began to increase 12 days after administration and reached a maximum 18 days. It was maintainedat a high level for 172 days or more. Contact infection by cohabitation was established up to 10 days after administration.
    Attenuated virus was recovered from the trachea, lung, and an emulsion of liver, spleen, and kidney, although it was small in amount.
    Attenuated virus was examined for recurrence of pathogenicity. The virus was successfully subjected to five passages in chickens. The resultant virus showed no enhancement in pathogenicity for chicks. Therefore, this virus is assumed to be available as strain for the preparation of live virus vaccine.
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 24 Issue 3 Pages 130-134
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.130
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    1971 Volume 24 Issue 3 Pages 147-155
    Published: March 20, 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.147
    JOURNAL FREE ACCESS
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  • Erratum to : Sensitivity of Freshly Isolated Strains of Erysipelothrix insidiosa to Antibiotics
    1971 Volume 24 Issue 3 Pages 165
    Published: 1971
    Released on J-STAGE: June 17, 2011
    DOIhttps://doi.org/10.12935/jvma1951.24.3_165
    JOURNAL FREE ACCESS
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