In antemortem examination, a cow was diagnosed as malignant edema caused by Clostridium perfringens. It seemed to have been involved in the wound infection type of this disease, although it had no apparent wounds in the skin or subcutaneous tissue. A possibility of infection through the pathway of parturition was denied from the observation on the uterus and the calf born. No stall infection was induced among 2 cows in the same stall, or 4 cows and 3 heifers in the neighboring one. It had never been reported from the abattoirs in Ibaraki Prefecture that any case of malignant edema had been detected from among cattle received for regular Slaughter, since this disease takes an acute course in these animals. Judging from the type of fever and other clinical signs, the detected cow was assumed to be in the progressive stage of disease.
Staphylococci and colon bacilli were isolated from lesions of various types of respiratory diseases, fowl pox, staphylococcosis, and colibacillosis in chickens over a period from September, 1963, to March, 1965. They were examined for susceptibility to sodium sulfamonomethoxine (MN), sodium sulfadimethoxine (DM), sodium sulfaphenazole (PH), and sodium sulfamerazine (MR) in vitro. A strain was regarded as a drug-fast one when its growth had been inhibited at a concentration of 100mcg/ml. Of 25 staphylococcal strains, 4 showed the fastness against MN, 5 against DM, 6 against PH, and 14 against MR. Susceptibility to MN and DM was higher than that to the other drugs in these strains. The 4 strains resistant to the action of MN had crossfastness against the other 3 drugs. Of 58 coli strains, about 70 per cent exhibited fastness against the 4 drugs and cross-fastness to them, too. The susceptible strains revealed a higher susceptibility to MN than to any other drug.
Pamaquine naphthoate, in the form of powder at 1: 20 concentration, was mixed with miso, or soybean paste for seasoning, and given to cows, which were soon accustomed to lick it. No side effects, except very mild fever, appeared when a daily dose of 6g per 100kg of body weight had been administered for 5 to 10 days. Small-sized Piroplasma organisms began to be expelled about 6 days after administration. They seemed to be almost exterminated at about 30 days. They appeared again at 60 days, but anemia showed a tendency to dissipate. Besides, no Babesia organisms were observed in any of the cows treated.