The sterilization effect of ethylene oxide gas (EOG) was examined on Bacillus subtilis var. niger in an LOG-sterilization locker containing carriers, such as filter paper, polyester fabric with 35% cotton blended, and cotton cloth, and expressed as D value. The following results were obtained.
1) B. subtilis var. niger showed a tendency to die a logarithmic death with an EOG concentration of 1, 2, or 3% at 30 or 40°C.
2) The registance to EOG was found in the following order: cotton>polyester>paper.
3) The registance to EOG of each carrier was reduced to one-half when temperature was raised from 30 to 40°C.
4) The gas treatment condition within 16 hours (at the inactivation factor 106) was as follows: The EOG concentration was 3% at 30°C and 1% at 40°C in polyester-cotton fabric, and 2% at 40°C in cotton cloth.
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