Some investigations on the relationship between the possession of antibodies (complement-fixing (c. f.) and neutralizing antibodies) in pre-inoculation serum and susceptibility for virus inoculation, were carried out with 2-to 4-month-old puppies. The following results were obtained.
1.(a) After the virus inoculation, neutralizing antibody was demonstrated more than c. f. antibody in mild or symptomatic cases.
(b) Severe and fetal cases were found mostly, among animals without both antibodies, although a few animals survived without them.
So, it seems that there are some relations between the neutralizing antibody and the susceptibility.
2. After the virus was inoculated in to puppies intravenously, both antibodies were not demonstrated in the course of disease in fatal acute cases, but in survived cases, development of these antibodies was recognized 7 days after inoculation.
3. In guinea pigs inoculated with the virus intraperitoneally, remarkable development of both antibodies was observed about 2 weeks after inoculation.
From these observations, it is shown that a guinea pig can be employed, instead of a dog, in the assay for vaccine and anti-serum of infectious canine hepatitis. That a dog is considered as only susceptible animal, constitutes
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