It was generally confirmed that microfilariae disappeared from the peripheral blood of infected dogs about two weeks after administration of antimony preparation. At this time, euglobulin (Eu) showed a decrease and pseudoglobulin I (PI) a marked increase in most cases. An increase in pseudoglobulin II was also observed as a transient phenomenon when microfilariae had been evacuated. These changes were exhibited correlatively in paperelectrophoretic patterns. In many cases, the increase of Eu, as salted out, observed at the time of evacuation of microfilariae was expressed as an increase in gamma-globulin ( γ-gl) and that of PI observed at the time of disappearance of microfilariae as an increase of β-gl and a concurrent decrease of γ-gl in these patterns. When medication was performed so as to destroy adult worms and microfilariae at the same time, Eu increased in accordance with the evacuation of adult worms into the pulmonary artery or the organization and absorption of the worm body. At this stage, microfilariae were seen in the experimental cases for a longer time than in those of a sigle administration of antimony preparation. Therefore, it was presumed that the Eu fraction might be a factor which would instigate microfilariae to swim into the peripheral blood. The relationship of absolute amount between the Eu and PI fractions was expressed as Eu<PI when microfilariae had disappeared completely from the experimental cases. The A/G index showed no remarkable changes when microfilariae had been destroyed. The total globulin, however, was found to have changed its composition in such case.
Nine piglets were used to study the anthelmintic effect of diethylcarbamazine (DC). Untreated contrl animals began to excrete worm eggs into the feces 28 to 30 days after experimental infection. Excreted eggs increased in number gradually and E. P. G. ranged from 200 to 1, 877 over the period of experiment. The lung harbored 167 to 238 wofms. In animals injected intramuscularly with10O mg/kg of DC daily for 7 or 10 days beginnin on the 14th day after infection (a. i.), the feces contained no eggs and the lung harbored no worms. The same results were obtained from anirnals having received the same daily doses for 10 days begining on the 7th day a.i., but not from those having received 7 daily doses, instead of 10.In the latter, egg excretion was observed continually during a period beginning on the 30 th day a.i. The E. P. G. was about one-tenth of that of the control. The lung had 98 parasitic worms. The anthelmintic rate was 49 per cent. A total of four courses of cyanacethydrazide treatment consisting of three consecutive dailysubcutaneous doses of 40 mg/kg each were given beginning on the 7 th, 14 th, 24 th, and 48 th days a.i., respectively. As aresult, changes in, E. P. G. were the Same as those observed in the untred Control, and the lung harbored 305 worms. Accordingly, hardly any effect was proved in this treatment. Growth of piglets was apparently more accelerated in the DC-treated group than in the control. It was confimled the refore that DC treatment had a very pfonounced anthelmintic effect upon swine
Bacteriological and pathological examinations were carried out on pathological materials (lung, liver, spleen, kidney, and heart) collected from an antelope (Japanese breed) which had been reared at a zoological garden and which died a natural death. The acid-fast bacilli isolated from this animal were identified as Mycobacterium tuberculosis. They showed eugonic growth on glycerinated egg media and give positive niacin test. Their growth was inhibited by potassium tellurate. They revealed a limited virulence in inoculation test on rabbits. Such pathological findings as characteristic of tuberculosis were observed in the lung, spleen, and liver. From these findings, a possible transmission of tuberculosis from a captive animal to man would be considered.