Respiratory and digestive disorders, including anorexia, pyrexia, coughing, and diarrhea, were observed in most cattle on a dairy farm in Kyoto, Japan. Bovine adenovirus-specific genes were detected by PCR and bovine adenovirus type 2 (BAdV-2) was isolated from the feces of several animals. A significant increase of BAdV-2 antibody titers was confirmed with a virus neutralization (VN) test using paired sera, suggesting the association of the virus with the clinical signs. A BAdV-2 seroepidemiological survey was conducted using 599 archive serum samples collected from cattle from surrounding farms in the previous 3 years. BAdV-2 VN titers were detected in 516 samples (86%). These results suggest that BAdV-2 is indigenous and widely spread in cattle in the region where the survey was conducted.

A 14-month old, male toy poodle with a history of pancytopenia was referred to our hospital. The dog was diagnosed with idiopathic aplastic anemia through laboratory examination, including bone marrow examination. Immunosuppressive therapy with prednisolone and cyclosporine, and cytokine therapy with granulocyte colony-stimulating factor and erythropoietin were successfully induced into remission on the 32nd day. Subsequently, the dog maintained a good condition and prednisolone was stopped on 89th day. As of the 395th day, remission has been maintained through the single administration of cyclosporine.

We measured haptoglobin (Hp) of some dolphins in the aquarium in Aomori Prefecture by modified hemoglobin binding assay. The mean Hp values of healthy and unhealthy bottlenose dolphins were significantly different (P＜0.01) at 2,119 μg/ml and 7,495 μg/ml, respectively. In the case of death from lung abscesses, Hp value increased to 8,157 μg/ml prior to a decline in A/G ratio and erythrocyte sedimentation rate. These results suggest that Hp is an inflammatory marker in dolphins and may be useful for early diagnosis of pneumonia.