Histopathological examination was carried out on 162 samples of reproductive organs, particularly the uterine tubr, collected from sterile, pregnant, and slaughtered cows since 1955 to clarify the relationship between sterility and diseases of the uterine tube. Various pathological changes of this tube were observed in 34 samples (42 uterine tubes). They occurred to the sterile cows at a somewhat higher frequency than the others. They were very mild in the pregnant cows. of them, edema was the most frequent, accompanied by swelling and deposition of fluid in the lumen of the tube. It was classified into three types, multi-acinous, uniacinous, and cystic. The first type was formed by adhesion of mucosal folds and interstitial hypertrophy. In the second type, the fimbriated end of the tube was closed to make the lumen enlarged with regressive folds. The third type was a cystic enlargement of part of the tube as a result of adhesion of mucosal folds. Most of these changes were complicated with ovarian and uterine disorders.
Chlorination with less than 1, 000ppm of chlorine was not effective at all against Salmonella contaminating the skin. Chlorination with 2, 000 ppm, however, showed a germicidal effect when performed simultaneously with agitation by a stirrer. Sensitization with 200ppm of chlorine water for 10 minutes was destructive to Salmonella contaminating feathers when the number of contaminating organisms was less than n×102. When it was carried out concurrently with agitation by a stirrer, it became so markedly germicidal as to kill n×104 organisms in 10minutes. When residual chlorine was 50-100ppm, it destroyed Salmonella contaminating water used for working in the dressing plant and cooling.
Four groups of 8×10 dairy cows each were selected from among those fed a ration composed mostly of concentrates (A), byproduct feeds (B), roughage (C), and relatively well-balanced concentrate and roughage (D), respectively, in the suburbs of Tokyo. Total digestible nutrient was a little smaller in amount in the groups other than B. Digestible crude protein was a little larger in amount in all the groups, except C. Butter fat rate and protein content were higher and solids-not-fat lower in the milk of group B than in that of any other group. The cows of group B were all positive for the California mastitis test. Serum protein, blood sugar, inorganic phosphorus, and calcium were high in group B. Blood sugar was especially low in group C. In urinalysis, the positive rate of ketone bodies was the lowest in group D.
The NS-175, 1185, and 1358 strains of virulent ILT virus were adapted to monolayers of primary chicken kidney cell cultures. Cultures of chicken embryo fibroblasts, bovine swine and canine kidney cells, and TC, PS and BHK-21 cell (line) swere resistant to the cytopathtic effect of the 11th passage of the NS-175strain. The peak titer of the NS-175, 1185, and 1358 strains was 103.5-6.5, 103.5-5.5, and 103.5-4.7 TCID50/0.1ml, respectively' in chicken kidney cell cultures. Systematic passage of the NS-175 strain in cell cultures resulted in changes in virus characteristics, which were manifested by a significantly decreased virulence of the virus for susceptible chickens. When inoculated ocularly and intranasally, vaccines prepared from the modifided virus (99th to 124th passage levels) provided good protection against challenge with virulent virus.