The rate of resistance to colistin (CL) in Escherichia coli (E. coli ) strains isolated from cattle in the Tokachi area of Hokkaido between 2010 and 2018 was 19.6% (9/46), and all resistant strains were derived from beef cattle without CL treatment. The results of plasmid transfer tests suggested that mcr-carrying plasmids were transferable with resistance to some antimicrobials other than CL in four strains. Twelve strains showing an MIC value of 2 mg/l or more to CL possessed mcr-1, mcr-3, or mcr-5, eight of which were Shiga toxin-producing E. coli O1, O26 and O111. In this study, CL-resistant pathogenic E. coli strains possessing mcr were detected in cattle; therefore, prudent use of antimicrobials on farms is required to prevent transmission of mcr-possessing strains to humans via livestock products.
We investigated bovine respiratory disease complex by collecting bronchoalveolar lavage fluid (BALF) for bacteriological and virological examinations from fattening cattle following their introduction from market, and monitored their treatment records for any subsequent history of medical interventions. A cohort of 10 steers underwent BALF collection in April 2016, and Pasteurella multocida and Mycoplasma bovis were isolated in samples from eight and one animal, respectively, in bacteriology, and Respiratory Syncytial (RS) virus genetic material was detected in a sample from one animal in virology. A second cohort of 10 steers from the same herd underwent BALF collection in December 2016, and Pasteurella multocida and Mycoplasma bovis were isolated in samples from four and one animal, respectively, in bacteriology, and RS virus genetic material was detected in samples from all 10 animals in virology. Monitoring post-introduction treatment showed that the cohort with ubiquitously detected RS virus tended to require earlier medical interventions. These findings indicate the presence of pneumonia-causing bacteria or viruses in the bronchoalveolar region of cattle after introduction, which may be important for the development of preventive measures and treatment strategies.
Tranexamic acid (TXA) is recognized as a highly safe emetogenic agent and is most commonly used for emetic treatment in dogs in Japan. However, we experienced two patients each with serious adverse events after TXA emetic procedure. One patient died of shock after the administration of TXA. Histopathological examination revealed thrombosis of the pulmonary artery, diffuse hepatic congestion, and renal amyloid deposition. The other patient developed status epilepticus after TXA administration, which improved with the administration of antiepileptic drugs for several days. A questionnaire was send to clinical veterinarians to ascertain the occurrence of adverse events after TXA-induced emesis. The results showed that 15% of veterinarians had observed such adverse events. The most common adverse event was convulsion.
In a Holstein cow, we found a case with different tumors in the thyroid, adrenal gland, colon and thoracic wall. Based on histopathological, immunohistochemical and molecular biological findings, the thyroid gland was diagnosed as having C-cell carcinoma, the adrenal gland as having pheochromocytoma, the colon as having a gastrointestinal stromal tumor and the thoracic wall as having B-cell lymphoma. Infiltration of the B cell lymphoma was found in the tumor tissue of the C-cell carcinoma. This case was the first report of four different tumors in a cattle.