A rabbit was immunized with γ-globulin purified from swine serum to produce a serum which showed a titer of 1: 128 in the precipitation test. Then γ-globulin was purified from this serum and labelled with FITC. Specificity was confirmed by using the labelled γ-globulin, free organisms of the RH strain, and antigen prepared from the intracerebral cyst of the Beverley strain. When the dye-testt titer was estimated by using Toxoplasma-positive swine serum, it was demonstrated that the Beverley strain gave rise to a stronger fluorescence. This labelled antibody was employed to compare the proposed method with the hemagglutination test. The results were almost the same between that method and this test. It is expected that the indirect fluorescent antibody method will be applicable to the diagnosis of toxoplasmosis more effectively if this method was standardized after solving some problems about it.
Atrophic rhinitis broke out on a hog farm in April, 1969. On this farm, 4 sows and 35 piglets were divided into 2 groups, an experimental and a control. In the experimental group, tylosin phosphate (20g/kg) and sulfisozole (20g/kg) were added to the ration at the rate of 0.5% At the same time a trial was made to clean the farm from Bordetella bronchiseptica. The medicated ration was given to sows for a week immediately before parturition and during the period of nursing and to piglets for 2 consecutive months after birth. 1. Pathological changes were a little milder in the experimental than in the control group. 2. The more advanced the turbinated bone was in the degree of atrophy, the less satisfactory became the growth of the piglet. Growth was more remarkable in the experimental group after 2months of age. At the time of shipment, this group showed an average gain in body weight 4.9kg greater than the control group. 3. The trial to free the farm from B. bronchiseptica was not successful, in spite of disinfection of pens, feeding of medicated rations, injection and intranasal instillation of drugs.
A total of 34 strains of Vibrio coli isolated from swine dysentery were examined for snesitivity to 31 antibacterial drugs, using the agar plate dilution method. They were incubated in an atmosphere containing 10% CO2. The results obtained are summarized as follows. All the strains tested were proved to have high sensitivity to nitrofurans, chlortetracycline, tetracycline, oxytetracycline, and chloramphenicol. Most of them were relatively sensitive to polymyxin-B, colistin, aminobenzyl penicillin S, sulfa drugs, andcarbadox. There were some strains resistant to sulfa drugs. Most of the strains were inclined to be decreasingly sensitive to penicillin G, thiophenicol, fradiomycin, and kanamycin in the order listed. They were almost insensitive to mikamycin, novobiocin, hygromycin, bacitracin, streptomycin, and dihydrostreptomycin. In addition, there were not a few of them which showed a cross-resistance to macrolide antibiotics.