Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 42 , Issue 1
Showing 1-8 articles out of 8 articles from the selected issue
  • KATSUMI HAMANA
    1989 Volume 42 Issue 1 Pages 29-38
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Download PDF (1667K)
  • HIROSHI ITOH, YOSHIKO MOTOI, KEIJI TAMURA, HIDEO MURATA, TSUTO CHIBA, ...
    1989 Volume 42 Issue 1 Pages 39-43
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Serum α1 acid glycoprotein (α1AGP) was examined in cows with bovine leukemia (BL), and compared with other diseased cows. Inhibitory effects of α1AGP isolated from a healthy cow and from a BL cow were also examined on the mitogenicity of healthy cow lymphocyte. In cows with BL and liver abscess, α1AGP was detected as several protein bands, not observed in healthy cows, at the position of isoelectric point (3.2-4.2) by plate electrofocusing. By radioimmunodiffusion, serum α1 AGP averaged 284.4±95.5 μg/ml for healthy female cows (less than 450 μg/ml in 95%) 316.7±101.3μg/ml for reared cows and 395.7±295.5 μg/ml for calves (those up to 14-day-old showed higher values compared with adults and reared cows). Cows with BL and other various diseases showed remarkably high α1AGP levels, 1021.6±1747.6 and 1116.7±858.7 μg/ml, respectively. In cows with BL, the following values showed positive correlation with serum α1AGP concentration: WBC count, lymphocyte count, sialic acid value and LDH level. The inhibitory effects of each mitogen, which was added with refined cow α1AGP and BL cow sera different α1AGP content, were revealed on the blastogenesis of healthy cow lymphocytes almost proportionally to the α1AGP concentration.
    Download PDF (2824K)
  • SATOSHI OSAME, SHIGERU ICHIJO, TAKUROH OOTANI
    1989 Volume 42 Issue 1 Pages 44-48
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Clinical and Clinicopathological observations were made on white muscle disease in four lambs of the Suffolk breed, 8 to 30 days of age in. Tokachi district, Hokkaido in March, 1986. Primary symptoms were recumbency, stiffness, tachycardia and cyanosis. Serum enzyme activities (GOT, GPT, CPK, LDH) increased markedly, as did LDH1 and LDH5. Electrocardiograms of three lambs showed sinus tachycardia, elevated or depressed ST segment, higher T wave, splitting of QRS complex and ventricular premature beat. Pathological examination indicated severe degeneration, of skeletal muscles and cardiac muscles under the endocardium of the right ventricle and interventricular septum.
    Consequently, measurement of serum enzyme activity, especially LDH isoenzyme, and electrocardiogram are useful diagnostic tools of white muscle disease in lambs.
    Download PDF (8638K)
  • HIROKAZU ITOH, SYOJI OKADA, HIROYUKI SUGIYAMA, IKUO KONDOH
    1989 Volume 42 Issue 1 Pages 53-57
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    On a farm in Aichi Prefecture, a disease with diarrhea broke out among 300 pigs one to two months of age, and 30 pigs died in October, 1978. Autopsy of three pigs revealed accumulation of yellowish watery contents in the intestines and inflamed intestinal lymph nodes. Histological examination revealed activation of the reticuloendothelial cells of the liver, spleen and intestinal lymph nodes. Bacteriologically, large amounts of Salmonella sp.(1) serovar infantis (6, 7: r: 1, 5) were isolated from all the organs and feces of pigs showing diarrhea.
    Susceptibility to drugs, such as CER, CL, GM, NA, OA and FZ, was demonstrated, as was resistance to PC-G, AB-PC, DSM, FM, CP, CTC, OTC, and SDM.
    Download PDF (745K)
  • KAZUE SAIJO, MINORU HIGASHIHARA, TADASHI YAMAGAMI, YUJIRO FUJISAKA
    1989 Volume 42 Issue 1 Pages 58-63
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Immunosuppressive potentials of attenuated variants (clones Lp and Sp) of infectious bursal disease (IBD) virus to the chickens were investigated comparing with those of virulent RF-1 strain (WT) and tissue culture-adapted RF-1 strain (the parent virus of the clones RF-1tc). After being orally inoculated with these viruses at one day or 21 days of age, the chickens were administered with either Newcastle disease B1 vaccine intranasally, Newcastle disease TCND vaccine intramuscularly or bivalent infectious coryza vaccine intramuscularly; these were administered at 7, 28 or 35 days of age, respectively. Hemagglutination-inhibition antibody titers were assayed at weekly intervals, and resistance to the challenge was tested 4-5 weeks later.
    Magnitude of immunosuppression due to the IBD virus varied depending on the virulence of IBD virus, the age of chickens at the time of IBD virus infection, and the sort of vaccine administered. The suppression was largest with WT, moderate with RF-1tc, and no suppression with clones Lp and Sp. Chickens inoculated with IBD virus at one day of age were remarkably suppressed, but those inoculated at 21 days were only slightly. Immunosuppressive effects against Newcastle disease B1 or bivalent infectious coryza vaccines were more severe than that against Newcastle disease TCND vaccine.
    The lack of immunosuppression after infection with clones Lp and Sp may reflect our previous results that the histopathological lesions due to the clones are less extensive than those produced by more virulent IBD strains. The results suggest complete attenuation of those clones.
    Download PDF (1096K)
  • EIKO OOKUBO, HIROYUKI OKADA, YUTAKA CHIHAYA, KIYOSHI MATSUKAWA
    1989 Volume 42 Issue 1 Pages 64-67
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Disseminated nodular neoplasms were found on the ceolomic serosae of two cows, and they were suspected macro-and microscopically to be malignant mesothelioma. After histochemical and electron microscopical investigations, one was diagnosed as intestinal adenocarcinoma, and the other as uterine adenocarcinoma. The disseminated nodular neoplasms on the coelomic serosa were confirmed to be metastatic lesions.
    Download PDF (7949K)
  • HIROYUKI SUGANO, TOHRU FUKASE, SHIRO CHINONE, HIROSHI ITAGAKI
    1989 Volume 42 Issue 1 Pages 68-71
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Nine poppies two to three months old, developed clinical symptoms of giardiasis three to six days after being purchased from a breeder in Matsuyama-shi, Ehime Prefecture, Japan, in July 1987. Giardian trophozoites were detected in their feces. When 25 adult dogs reared in the facility of the same breeder were monitored, 18 dogs (72%), including the mothers of the infected puppies, were positive for cysts in the feces in spite of their normal appearance. Those results suggest that the poppies had been infected with giardian parasites when they were raised with their moth ers, and the stress of separation from the mothers may evoke clinical symptomology.
    Metronidasole (Flagyl®, Shionogi & Co., Ltd., Osaka, Japan) was orally administered at a daily dose of 60 mg/kg for six consecutive days to all nine puppies and six of the 18 adults from which giardian parasites were detected. Consequently, giardian organisms were completely eliminated from all the puppies and five adults. One adult dog remained a carrier, but fecal cysts were markedly decreased relative to those before treatment.
    Download PDF (3093K)
  • MASATAKE KIKUCHI, KIYOSI MEMIDA, TETSUYA OSAWA, MASARU YAMAZAKI, MITSU ...
    1989 Volume 42 Issue 1 Pages 72-74
    Published: January 20, 1989
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    The utility of the enzyme linked immunosorbent assay (ELISA) was assessed for detection of antibody to Chuzan virus, by using positive antigens obtained from BHK-21 cells infected with Chuzan virus and negative antigens from non-infected BHK-21 cells, through processing with Nonidet P-40.
    For this assay, 1/100 diluted non-inactivated serum and 1/200 diluted enzyme-labeled antibody were prepared, and the difference in their OD values for each antigen was regarded as the ELISA value of the serum. When the serum giving ELISA value of 0.200 or more was supposed to be antibody-positive, a close correlation between ELISA antibody and neutralizing antibody was observed (r=0.83, y=-0.124+0.33x).
    Download PDF (452K)
feedback
Top