Chicks exhibiting dorsal backward bending of the neck, such as opisthotonus, were successively bred, and the third generations of bred chicks and embryos prior to hatching were pathologically examined. The incidence of clinical signs had increased notably, rising to 62.5% by the breeding. Histopathologically, edema between muscular fascicles, variability in size, and hyalinization of muscle fibers and myophagocytosis were observed in a significant percentage of cases, not only in the complexus muscle but also in other skeletal muscles, especially in the cervical region. These results suggest that this disorder is hereditary. Since the pathological lesions were limited in the skeletal muscles, the characteristic clinical signs could potentially be caused by these muscular changes.
Eight Japanese Black breeding cows in a farm holding 120 cows showed anorexia, discouragement, swelled pastern and lameness within a few days after changing roughage to tall fescue straw imported from the United States. One cow of the five severely affected animals the hoof capsule of its right hind leg. The suspected straw contained 1,200μg/kg ergovaline, while the level of lolitrem B was below the detection limit (50μg/kg). Nitrate nitrogen concentration of the straw was 630 mg/kg. Anorexia and discouragement were also observed in the cattle at one of the eight farms using tall fescue straw from the same shipment. From these observations, we diagnosed this case as fescue foot, although experimental feeding of the suspected straw to Japanese Black cows failed to reproduce clinical signs observed in the field case.
In this study, ophthalmic examinations including a Schirmer tear test and pathological examination of the ocular tissues, including the lacrimal glands, were carried out in two dogs with corneal ulceration. The two dogs were diagnosed with Keratoconjunctivitis sicca (KCS) based on the clinical findings. Inflammatory cell infiltration, dominated by lymphocytes, was observed in the interstitium of the lacrimal gland tissues. Occasional destruction of the acinus structure was associated with the lesions. Association of the immune-mediated inflammatory changes in the lacrimal gland tissues is suggested as one of the underlying factors responsible for the development of canine KCS. Further study is required for elucidation of possible association of the auto-immune mediated mechanism, which is similar to Sjögren's syndrome in humans, with the observed changes in the present two dogs.
It was recently reported, on the basis of a taxonomic study, that some strains belonging to Erysipelothrix tonsillarum serovar 7 are a causative agent for dogs with endocarditis. In the present study, to search for the epidemiological features of the infection among dogs, we surveyed the levels and distribution of anti-Erysipelothrix antibodies by way of a growth agglutination (GA) test on abandoned dogs. The serum samples used in this study were obtained from 166 dogs at the Hyogo Prefectural Animal Well-being Center, from 2006 to 2007. The results of the GA test serological survey are as follows. In total, a GA titer of 1 : 16 or higher, which is considered to be positive, was detected in 7 (6.6%) of 106 serum samples, with a high titer of 1 : 128 for one sample, and in 7 (11.7%) of 60 serum samples from the dogs in 2006 and 2007, respectively. In comparison with that from fiscal year 1999, the present positive rate showed a tendency to increase annually. The existence of dogs with a positive level of antibodies against Erysipelothrix with an average of 8.4% prevalence, even if this is a low proportion, indicates a certain risk of Erysipelothrix infection among household dogs.
A six-year-old male rabbit presented to the hospital primarily exhibiting lack of appetite was diagnosed via x-ray with cystic calculi. In the urinary bladder, an edematous lesion was seen when the gaster was cut open for lithectomy. A hydroureter with no accompanying uronephrosis was accepted. The cystotomy approach was made dorsally. Carrying out tylosis of the tunica mucosa vesicae urinariae, calculi were found to be pressing against the ureter orifice of the urinary bladder trigone. The extracted calculi were calcium carbonate.
We investigated the availability of the portable endotoxin test system by the chromogenic method for milk hygiene. We measured endotoxin concentrations of commercial milk and milk drinks by the portable endotoxin system and conventional system of the kinetic turbidimetric method. We also evaluated the correlation and regression between the values of endotoxin concentrations using both methods. The endotoxin concentration values were strongly correlated (R ＝0.99), and a good linear regression was recognized for those logarithms. Therefore the portable endotoxin test system may be able to measure endotoxins in milk and milk drinks.