It is an ideal general, anesthesia when it is produced by a simple method of application without any side effect and maintains its effective, deep anesthetic stage as long as possible within a necessary extent. In order to produce anesthesia, it is rather infrequent at present to use only one of various anesthetics singly, but there is a tendency to administer some kinds of drugs simultaneously. In such cases, attention must be paid to the pharmacological characteristics, specificity of method of administration, and side effect in practical use of each anesthetic. Failure in anesthesia may not only deal a fatal blow to the subsequent clinical treatment, such as operation and therapy, but also lead animals to death frequently. In clinical circles where there is always a problem how general anesthesia can be established precisely so as to facilitate succeeding treatment, it is absolutely necessary to determine any method of administration of anesthetic by the intravenous route with much safer and more effective results than any other method frequently employed at present. From this intention, the authors conducted a series of experiments. As the interference with respiratory movement was pointed out as the most dangeous side effect of anesthetic introduced by the intravenous route, it was examined by electromyography. The resulting electromyograms were used for judgment. From the results of experiments with single administration of rabonaru, a proprietary product of barbital derivative, it was made clear that the safest, most effective method consisted in injecting 25 mg/kg of this anesthetic by spending at least 2 minutes for the whole dose.
Dichlorophen compounds were examined for anthelmintic efficacy on Raillietina cesticillus and effect on egg production of hens. (1) A preparation containing 300mg/kg of dichlorophen and 300mg/kg of phenothiazine was given to 5 Lockhorn cockerels experimentnally infected with R. cesticillus without previous starvation (Table. 1). No tapeworms were found in their small intestines, except some ascarids which were found at postmortem examination made 4 days after the medication (Table 3). (2) A preparation containing 120-150mg/kg dichlorophen and 120-150mg/kg phenothiazine was given to 8 White Leghorn and New Hampshire (laying hens) naturally infected with R. cesticillus and R. echinobothrida (Table 4). As a result, it did not have 100per cent anthelmintic effect on tapeworms. This preparation was given to 10 birds 7 days after the first medication (Table 4), but it did not exercised 100per cent anthelmintic effect on tapeworms. (3) A preparation containing 120-150mg/kg of dichlorophen, 100-130mg/kg of phenothiazine, and 50-80mg/kg of piperazine sulfate was administered to 6 birds in which the first and the second medication had failed to remove tapeworms. As a result, no segments were found in the droppings of all the birds, except one, for 26 days after the medication. (4) Administation of this preparation did not interfere with the egg production of laying hens. (5) It is obvious that the preparations containing dichlorophen, phenothiazine, and piperazine were effective in removing R. cesticillus and R. echinobothrida.
Experimental studies were made on various media for the detection and counting of lactic bacteria in fermented milk and acid milk drink. The results obtained are summarized as follows. follows. 1) Tomato juice agar, lactic agar, and plate count agar had excellent qualities for the detection and counting of lactic bacteria.When materials contained bacilli and cocci, it was difficult to make differential count macroscopically. 2) Lactic streptococci developed selectively on Delta's fuchsin lactose agar.The growth of lactic bacilli and other bacteria was controlled on this medium. In conclusion, it is recommended that tomato juice agar, lactic agar, and plate count agar be used for counting total lactic bacteria and that Delta's fuchsin lactose agar be employed to distinguish bacilli from cocci
From experimental results, it was found that the following care was needed for manufacturing fish-meat sausage. 1. In manufacturing fish sausage, pulverized spicery should be sterilized thoroughly before use, or sterile fluid spicery should be employed. 2. Metal-packed sausage is more air-tight than string-fastened sausage. 3. Inhibition of bacterial growth is more remarkable in sausage containing nitrofurazone than in sausage to which sorbic acid was added, either in the immersing process of manufacture or in keeping quality tests.
The authors detected a sick cow, the clinical and histopathological findings of which were completely identical with the description of listeriosis published in Europe and Americas. The microorganisms isolated from its brain had characteristics identical with those of Listeria and caused monocytosis when inoculated into rabbits and conjunctivitis when dropped in the eyes.