Acute pneumonia was induced in three Holstein calves by intramuscular injections of corticosteroid, intratracheal nebulization of 8% acetic acid and intratracheal inoculation of Pasteurella haemolytica. Animals were examined for physical conditions, blood cytology and chemistry and X-ray. Ultrasonography (USG) was conducted from the 2 nd to 9 th intercostal spaces of the both sides using real-time B-mode linear scanners with 3.5 and 5.0 MHz transducers. At necropsy, the lung was scanned with a probe directly placed on the organ surface, and USG was also applied to excised lung samples, being soaked in a tank filled with air-freewater. The clinical and postmortem ultrasonograms and gross and microscopic pathology were comparatively studied. USG revealed an air echo pattern at the first stage of pneumonia followed by three types of changes with time; 1) Transfer of the air echo area to a low-level echo, 2) extension or no extention of the area, and 3) reduction or disappearance of the area. USG was suggested to be applicable to the diagnosis of acute pneumonia. Key words: acute pneumonia, air echo, calf, low-level echo, ultrasonography.
A 6-year-old Japanese Black cow suddenly showed hemoglobinuria and high level of blood urea nitrogen in serum and N-acetil-β-D-gulucosaminidase in urea as well as high values of leucocytes. Bacillus pumilus was purely isolated from the urine. The cow recovered on day 38 of illness after 15 intramuscular and intravenous injection of PCG and SM.
Pregnant Japanese Black cows were fed diets supplemented with selenium (Se) and vitamin E (VE) from 1 month prepartum to 1 month postpartum, and their calves, were examined for serum Se and VE levels, NBT reduction ability of neutrophils, antibody titers to enterotoxigentc E. coli (K99) and growth. The serum Se and VE levels in dams fed Se and VE and their calves were significantly higher than those in controls. Calves from dams fed supplemented diet showed greater NBT reduction ability 1 month after birth, and their serum antibody titers to K99 fimbriae tended to be higher. They showed greater tendency in daily gain (DG). Serum Se and VE concentrations in diarrheic calves and their dams tended to be lower.
A 19-day-old female Holstein calf weighing 37 kg showed eyeball protrusion and no sucking. From the results of Electroretinogram (ERG) and visual evoked potentials (VEP), ERG of the right eye showed a disappearance of a-wave and a prolongation of the peak latency of b-wave, and VEP of the both eyes showed low amplitude in the peak of long latency. Autopsy revealed that the case had hasilar abscess resulting from otitis media.
The plasma concentrations of chlormadinone acetate (CMA) in female cats were determined by GC-MS with the quantitaion limit of 0.2ng/ml. After intravenous injection of CMA solution, the half-life was 27 hr. After subcutaneous implantation of pellet (TZV-802) in female cats, plasma CMA concentration was maintained during 28 days, showing a half-life of 138 hr. The bioavailability of pellet implantation was about 100%.
Fifteen cases (8 males and 7 females) of feline acute leukemias, 11 months to 19 years (mean 4.1 years) of age, were studied clinicopathologically. According to the human French-American-British (FAB) classification, they were diagnosed M1 (1 case), M2 (5 cases), M4 (2 cases), M5a (2 cases), M6 (1 case) and L2 (4 cases). Three, 7 and 5 of the 15 cases were leukemic, subleukemic and aleukemic, respectively. Anemia was observed in all cats, and the causes of that were due to erythroid hypoplasia (12 cases), ineffective hemopoiesis (2 cases) and chronic inflammation (1 case). Neutropenia (9 cases) and thrombocytopenia (7 cases), decrease of megakaryocytes (11 cases) and increase of eosinophil in bone marrow (3 cases) were seen. AST levels were higher in 4 cases of M4 and M5 than 6 cases of M1 and M2 (p<0.05) and 4 cases of L2 (p<0.05). LDH levels were higher in cases of M4 and M5 than those of M1 and M2 (p<0.05). Survival times were longer in cases of acute lymphoblastic leukemia than those of acute non-lymphoid leukemia (p<0.01).
In a slaughterhouse, S. aureuswas isolated from 27% and 2% of the surface samples of washed swine and bovine carcasses, respectively, and from 4.3% and 1.4% of the swine and bovine intestinal contents, respectively. The isolates were of coagulase type VII from bovine carcasses, types I and IV from swine intestinal contents, and type VI from bovine intestinal contents. The organism was isolated from 5.7% of swine septicemic cases and all the isolates were of coagulase type IV. ByS. aureusslide latex agglutination test 20% of the isolates were negative. No isolates produced enterotoxins nor toxin-1, and all the isolates were sensitive to methicillin.