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Volume 69, Issue 4
Displaying 1-3 of 3 articles from this issue
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Farm Animal Medicine and Animal Health
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Tsuneyuki MASUDA, Tomoko ASHIBA, Hiroshi YAMASATO, Ken-ichiro KAMEYAMAArticle type: Original Article
2016 Volume 69 Issue 4 Pages 187-191
Published: April 20, 2016
Released on J-STAGE: May 20, 2016
JOURNAL FREE ACCESSIn order to prevent the spread of bovine viral diarrhea virus (BVDV) in public pastures, since 2013 all heifers in the Tottori Prefecture have been tested for BVDV using reverse transcription polymerase chain reaction (RT-PCR) before they are pastured. However, because RT-PCR is time consuming and labor intensive, a new commercial ELISA based on the detection of the Erns antigen was evaluated as an alternative method. Twenty-two sera of persistently infected (PI) cows and two paired sera of transiently infected (TI) cows were tested to evaluate sensitivity. All sera of PI cows were positive, and paired sera from the TI cow were negative. Field samples of 646 sera, including one PI cow, were tested for BVDV antigen using ELISA. Only one sample from the PI cow tested positive. However, it displayed a negative result when the PI serum was mixed with a high BVDV antibody-positive serum. Therefore, although performing ELISA on PI calves in the presence of persistent maternal antibodies requires attention, it is a useful screening test for detecting BVDV because of its high sensitivity and ease of use.View full abstractDownload PDF (664K)
Small Animal Medicine
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Hiroshi TANAKA, Masahiko KITAMURA, Manami KURIYAMA, Yuki MATSUMOTO, Yo ...Article type: Original Article
2016 Volume 69 Issue 4 Pages 193-198
Published: April 20, 2016
Released on J-STAGE: May 20, 2016
JOURNAL FREE ACCESSIn a study of small-breed dogs with suspected cervical intervertebral disc disease that was conducted between 2004 and 2014, the findings of 74 dogs diagnosed with compressive myelopathy through the use of myelography were reviewed retrospectively. Dynamic lesions were observed on 12 (16.2%) of the dogs examined. Compared with static lesions, dynamic lesions were commonly seen on non-chondrodysphoid dogs. In addition, in cases involving multiple lesions, the incidence was higher in the region with vertebral deformation/spondylosis deformans or the adjacent site.View full abstractDownload PDF (798K)
Veterinary Public Health, Wildlife and Environmental Conservation
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Masayuki KACHI, Kayoko MATSUO, Mikio KATO, Tokuma YANAI, Hiroki SAKAIArticle type: Short Communication
2016 Volume 69 Issue 4 Pages 199-202
Published: April 20, 2016
Released on J-STAGE: May 20, 2016
JOURNAL FREE ACCESSWhite masses were detected in the lungs and abdominal and thoracic lymph nodes of a 63-month-old-female Holstein cow in a meat inspection. Following a cytological examination, sarcoma with multinucleated giant cells was diagnosed using the stamp-smear method. Histopathological examination revealed that the tumor comprised a predominant proliferation of small round neoplastic cells with scattered myoblast- or myotube-like neoplastic cells. Thus, it was diagnosed as an embryonal rhabdomyosarcoma. Immunohistochemical analysis revealed that the neoplastic cells were positive for vimentin and desmin, the predominant small round neoplastic cells were positive for MyoD1, while the neoplastic cells with striated muscle differentiation were positive for myogenin. The present case is a rare form of bovine embryonal rhabdomyosarcoma. Moreover, multinucleated giant cells corresponding to the myotube-like neoplastic cells in the histopathological specimens were detected in the stamp smear.View full abstractDownload PDF (1171K)
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