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Volume 67, Issue 5
Displaying 1-6 of 6 articles from this issue
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Farm Animal Medicine and Animal Health
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Tsunehiko AITA, Hitoshi MOUE, Kazunori MURAYAMA, Satoshi OTAKE, Kensu ...Article type: Original Article
2014 Volume 67 Issue 5 Pages 323-327
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSFrom 14 swine herds in Niigata prefecture, serum and pen-based oral fluid samples were collected from 50 pig groups aged two to eight months. The specimens were assayed for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection using the indirect fluorescent antibody test (IFAT), ELISA and RT-nested PCR. With both IFAT and ELISA, 40 groups were positive and 10 were negative in the serum samples, and 38 groups were positive and 12 were negative in the oral fluids (96% matched). In terms of the detection of the PRRSV specific gene using RT-nested PCR, 18 groups were positive and 32 groups were negative in the serum samples, and 15 groups were positive and 35 groups were negative in the oral fluids (90% matched). Oral fluid sampling may contribute to the development of high efficiency, low cost PRRSV surveillance in pig groups.View full abstractDownload PDF (981K) -
Tomohiro IZAWA, Ken-ichiro TANAKA, Sei-ichi KAKINUMA, Yuka SUGIYAMA, K ...Article type: Original Article
2014 Volume 67 Issue 5 Pages 328-332
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSTo clarify the immune status of Japanese Black (JB) cattle infected with bovine leukemia virus (BLV), the peripheral leukocyte population and immune-related factors were analyzed. Thirty-two JB cattle were used for this investigation, and these cattle were divided into three groups: cattle with enzootic bovine leucosis (EBL) (EBL group, N=9), clinically healthy cattle infected with BLV (Carrier group, N=13), and clinically healthy cattle not infected with BLV (non-Carrier group, N=10). Leukocytes were analyzed for cell surface antigens as well as mRNA expressions of cytokines, pertorin, granulysin, MX1 and MX2. The number of WC1-N1＋ T cells in the EBL group was significantly lower than in the non-Carrier group, but the number of CD14＋ cells and MHC class-II＋CD14－ cells in the EBL group was significantly higher than in the non-Carrier group. The levels of perforin and MX-2 mRNA in the EBL group were significantly lower than in the non-Carrier group. These results suggest that there are decreased peripheral γδT cells in terms of number, and cytotoxic and antivirus functions might be reduced in JB cattle with EBL.View full abstractDownload PDF (275K)
Small Animal Medicine
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Yasuhito KUWAHARA, Akemi ISHINO, Norie KUWAHARA, Tetsuya KAWASAKI, Nao ...Article type: Original Article
2014 Volume 67 Issue 5 Pages 333-339
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSTwenty-six cats with pyelectasia, including 16 cats in which a calculus in the ureter on the pyelectasia side was detected, and 10 cats without a ureter calculus but diagnosed with hypercreatininemia, underwent the insertion of ureteral stents via laparotomy. Despite the presence of stenosis in the ureter, the stents were able to be inserted into the ureters of all the cats. After the insertion of the stents, the pyelectasia improved in all the cats. Following the treatment, six of the cats died, but the other 20 cats showed an improvement in azotemia, or did not show any acute exacerbation of chronic renal failure. We can consider the insertion of ureteral stents to be one of the viable treatments in cats with detected pyelectasia and ureter calculus on the pyelectasia side, or in cats with hypercreatinemia induced by the suspected ureteral stenosis.View full abstractDownload PDF (403K) -
Kazuhiro WATANABE, Yoshitaka KATOH, Mihoko HIRAYAMA, Sanae SHIBATA, Hi ...Article type: Short Communication
2014 Volume 67 Issue 5 Pages 340-344
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSA border collie (male, nine years and five months old) presented with left infraorbital swelling and severe calculus deposition on the left maxillary fourth premolar. An oral examination revealed presence of gingival swelling around the left maxillary fourth premolar and drainage from the buccal gingiva located around the distal root apex. Radiography revealed bone resorption around the distal root of the third premolar and the medial and distal root apexes of the fourth premolar in the left maxilla, and a deformity of the distal root of the fourth premolar. Based on these findings, the dog was diagnosed as having internal dental fistula caused by periapical lesions and underwent extraction of the left maxillary third and fourth premolars. The fourth premolar had four roots, and there was a fissure in the crown. Histological examination showed inflammatory cell infiltration in the gingiva, pulp necrosis of the fourth premolar, and bacterial colonies around the lesions. The supernumerary root had fissures in the crown. These fissures may have served as the path for the infection in the root apexes. Abnormal morphological changes such as supernumerary roots and fissures in the crown may contribute to the onset of periapical lesions.View full abstractDownload PDF (1269K)
Veterinary Public Health, Wildlife and Environmental Conservation
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Motohiro HORIUCHIArticle type: Review
2014 Volume 67 Issue 5 Pages 345-353
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSDownload PDF (403K) -
Kazuaki ONOArticle type: Original Article
2014 Volume 67 Issue 5 Pages 354-359
Published: May 20, 2014
Released on J-STAGE: June 20, 2014
JOURNAL FREE ACCESSThe aim of this study is to develop for the high sensitive isolation methods for Vibrio cholerae from frozen food samples. After being frozen at －20℃ for one week, the bacterial numbers of V. cholerae in physiological saline were reduced by a factor of 1/100-1/10,000. However, this bacterium was able to be isolated from the frozen food samples by using the immunomagnetic beads technique. In order to achieve a higher level of detection, enrichment procedures were repeated several times. A shrimp sample stored at －80℃ for one year after inoculation with 0.5ml (103 cfu/ml) of V. cholerae serotype O139 required the enrichment procedures to be repeated three times for isolation. In addition, an original selective agar plate supplemented with 0.1 % volume of sodium pyruvic acid and 2,000 U/plate of catalase was useful for the isolation of V. cholerae. In this study, the application of the immunomagnetic beads separation technique and use of the improved selective agar plate after the enrichment procedure of the samples (occasionally, repeated several times) should facilitate a comprehensive approach to the detection of small numbers of V. cholerae in frozen foods.View full abstractDownload PDF (279K)
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