When injected with 10ml of E-SE twice at a 2 weeks' interval, a pregnant cow showed an increase in a serum total tocopherol level for a short time. When administered orally with 50g/day of a premix, it presented no increase in serum total tocopherol levels. When injected with E-SE, the cow exhibited an increase in serum selenium level earlier and more exactly the cows administered with the premix. Blood glutathione peroxidase (GSH-Px) activities in calves born from cows injected with E-SE were higher than in those born from cows administered with the premix. premix. In calves injected with 250mg of α-tocopheryl acetate, serum tocopherol levels increased, but serum GOT and CPK activities decreased. In calves injected with 100mg of α-tocopheryl acetate, serum tocopherol levels did not increase, but serum CPK activities increased markedly. Calves showed an increase in serum tocopherol and selenium levels and blood GSH-Px activities, when injected with 2ml of E-SE.
Zymosan-activated serum showed a significant chemotactic activity, whereas formyl peptide f-MLP failed to attract bovine neutrophils. An optimal number of neutrophils and incubation period in the agarose method were 1×106 neutrophils and 2 hours, respectively. A significantly reduced chemotactic response of neutrophils in cows was found immediately after the parturient period, as compared with that in any other period. This reduction seemed to be related to the elevated cortisol concentration in blood during the parturient period.
In 27 mongrel bitches be reproduced by inoculation of Escherichia coli was inoculated into the uterus with the cervix ligated. pyometra was detected in all the bitches by laparotomy 3 days after inoculation. The removal of cervical ligature resulted in a decline in the incidence of the disease 12 days after inoculation. The decline rate was the highest in postpartum, higher in anestrus, and low in proestrus + estrus and diestrus.
Antibodies against Toxoplasma gondii (Tp) were examined by the Latex agglutination test (LAtest) in free living wild animals which had been caught in and around Kobe, Hyogo Prefecture, because of being wounded or diseased. The sera of cats (Felis catus) and rats (Rattus norvegicus) caught in the Oji Zoo were also examined for Tp titer. As a result, 7 of 19 specimes (37%) from mammals, 2 of 77 specimens (3%) from birds, and 0 of 2 specimens (0%) from reptiles presented a Tp titer of over 32. This titer was under 1: 16 in 17 cats an 15 rats caught in the zoo.
In May, 1985, a Holstein cow suffered from nervous symptoms and was condemned within a day. Pathological examination revealed local necrosis, degeneration of nerve cells and perivascular infiltration mainly in the brain stem. Listeria monocytogenes type 4b was isolated from the brain stem. Simultaneously, the same bacteria were isolated from the deteriorated silage feeded. This result showed good agreement with the isolated from the cow biochemically and serologically. Consequently, the silage seemed to be responsible for the disease.
Ovicidal activity of iodine was observed in the acid solution of iodine and a 50% growth inhibtion dose (GID50) ranged from 9.4 to 16.8μg/ml. It was hardly observed in the alkaline soultion. In the test of an anion of various salts, an increase in activity of iodine was observed in the following increasing order: chioric, sulfuric acid, citric acid and phosphoric acid ion. ion. Moreover, an increase in activity of iodine was observed in the test of an anionic surface active agents, such as sodium lauryl sulfate, sodium lauryl benzenesulfonate and sodium dialkylsulfosuccinate, as well as in the test of the acid soultion. GID50 ranged from 18.0 to 22.0μg/ml. An increase in activity of iodine on Ascaris eggs was observed in the test of sorbitane mono laurate (T-20) and stearate succharose fatty acid ester 1570 of 15 nonionic surface active agents examined. No activity of iodine was observed on cationic surface active agents.
The hemagglutination inhibition (HI) test with mouse red blood cells was used as a diagnostic method for detection of infectious bovine rhinotracheitis (IBR) virus (bovine herpesvirus type 1) infection in the field. IBR virus hemagglutinated C57BL/6 mouse red blood cells most clearly of five mouse strains tested. Bovine sera were collected from herds where IBR infection was observed. All sera which were treated with 10% mouse red blood cells after inactivated at 56°C for 30 min failed to show any nonspecific hemagglutination with red blood cells. Antibody titers determined by this test were slightly higher than those by the serum neutralization test. The obtained by both tests were highly correlated (r =0.778). The HI test was so sensitive that it could detect antibody in the early stage of infection. Therefore, it is considered that the HI test is a valuable method for the diagnosis of IBR virus infection.
A piece of hepatic tissue about 5 cm in length and 100-150 mg in wet weight could be collected by a single hepatic puncture. It was 2-2.5 times as large as that obtained by the conventional technique. Moreover, only a barely visible trace was left at the site of puncture in the liver. Histologically, red blood cells and inflammatory cells were found to have infiltrated into a region where tissue deficiency had been caused by puncture. This infiltration was limited to a single hepatic lobule. It did not extend to any adjacent hepatic lobule at all.
An anomalous coronary sinus emptying into the left and right atria was observed in an 8-monthold female Japanese black calf. The V. cordis magna, V. ventricularis sinistra posterior (V. cordis caudalis) and V. cordis media drained normally into the coronary sinus. The left end of the coronary sinus opened into the left atrium through an oval orifice 1 cm in longer diameter, while the right atrial ostium of the coronary sinus was completely obstructed. The four Vv. cordis dextrae from the lateral wall of the right ventricle drained into an anomalous common stem above the coronary groove. The posterior part of this stem was continuous with the coronary sinus and the anterior part opened into the right atrium through an oval orifice 3 mm in longer diameter.