Eggs laid in the same hen-house on the same day were divided into there groups: a washed one, a vaseline-coated one preserved at 5, 10, or 37°C, for two weeks and a non-treated one preserved at room tempaerture (27-32°C), 5, 10, or 37°C for two weeks.
1) Viable bacterial count per gram of egg content was lower in the vaseline-caoted group than in any other experimental group. Viable bacterial count increased in the washed and the non-treated groups in this order, and in proportion to a rise temperature.
2) Isolated were Streptococcus (lactic group), Micrococcus, Escherichia (E.
coli and E.
interrnedia), Staphylococcus (St.
epidermidis), Flavobacterium, Pseudomaons, Achromobacter, and Bacillue (
B. subtilis and B. megathelium).
3) Micrococcus and Streptococcus were isolated from all experimental groups at a count of 10
2.0-4.0 and 10
0.7-3.7, respectively.
4) Staphylococcus was isolated from eggs preserved at room temperature un-treated eggs at 37°C, and washed eggs at 10 and 37°C.
5) Enterococcus was isolated at an extremely high rate, in spite of its low viable count.
6) Flavobacterium and Achromobacter were isolated generally from eggs at low temperature. Pseudomonas was isolated at various temperatures.
7) None of anaerobic bacilli were isolated from any group.
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