Thirty cows with left or right side displaced abomasum were examined before and 7days after laparotomy Hemoconcentration, hypochloremia, hypokalemia and hypocalcemia were observed in them before operation, as well as an increase in chloride, a decrease in sodium and total VFA levels in ruminal fluid, and an increase in total VFA level, particularly butyric acid, in abomasal fluid. These changes were regarded as the results of anorexia and reflux to the rumen or absorption disorder of accumulated abomasal fluid. The hypofunction of the liver was found after operation.
Seventy-five cows with ovarian follicular cysts were randomly assigned to two treatment groups, A and B. Group A of 31 cows was injected intramuscularly with both 100μg of GnRH analog (GnRH·A)(fertirelin) and 25 mg of PGF2α THAM salt at an interval of 10 to 14 days. Group B of 44 cows was given 100μg of GnRH·A and held under observation for the occurrence of normal estrus, except those cows considered to have not responded to GnRH·A and retreated with 200μg of GnRH·A 10 to 14 days after the previous treatment. Progesterone concentrations in defatted milk were determined by ETA, and used for differential diagnosis of follicular and luteal cysts, and monitoring the luteinization of follicular cyst after GnRH·A treatment and the regression of luteinized cysts following PGF2α treatment. Of 6 cows of Group A and 38 Group B cows of defined to have follicular cysts on the basis of milk progesterone levels, 20 cows (76.9%) and 18 cows (47.4%) respectively, responded to GnRH·A with luteinization. Progesterone level in defatted milk decreased to less than 1.0 ng/ml within 6 days after PGF2α injection in 20 (100 %) of the 20 cows of Group A and 16 (80 %) of them exhibited behavioral estrus. The Percentage of cows inseminated within 100 days after the first treatment was significantly higher (P<0.05) in Group A 95%, 19/20 than in Group B 44.4%, 8/18. Average intervals in days between the initial GnRH·A treatment and the first AI were 19±9 days in Group A and 39±26 days in Group B. The difference was statistically significant (P<0.05). The percentage of cows which conceived within 100 days after GnRH·A treatment and the average interval between treatment and conception were 65%(13/20) and 33±19 days respectively, in Group A, and 40%(6/15) and 44±26 days in Group B. These results indicate that treatment with PGF2α of cows with luteinized cysts following treatment with GnRH is effective enough to shorten an interval between treatment and conception.
A portable echographic apparatus (Hitachi model EUB-200) in which modes M and B are recorded simultaneously was used. To raise the degree of resolution of a picture produced by echography, a video casette recorder for home use was remodelled into a super high band and used with the apparatus. Then, echography was carried out in clinical cases of canine filariasis, canine mitral regurgitation, canine myocardial infarction accompanied with endocarditis, and feline myocardosis. Each picture obtained from it was reproduced into a slow film for scrutiny. As a result, it was possible to locate the site of parasitization even with a small number of filariae rather accurately. The new method was effective for the diagnosis of valvovelar escape in mitral regurgitation and for morphological observation of the heart in myocardosis. It failed, however, to find any characteristic change in the case of endocarditis or myocardial infarction.
A method was developed to use radiography and endoscopy on the same day. Its utility was examined in gastric models of an elevated, an ulcerative and an inflammatory lesion. A contrast mixture of 50W/V% barium sulfate and Gastrografin (1: 1) gave a fine result for the detection of these lesions.
A total of 63 dogs kept outdoors were subjected to study the prophylactic effect of ivermectin against heartworm infections. They were medicated with ivermectin from July through December of 1984 and were negative for microfilariae. Each dog was given 6-9μg/kg of ivermectin orally once a month over a period from July 1985 to December 1985. All the dogs examined in May or June 1986 were negative for microfilariae. No side effects of ivermectin were found.
A 50% flubendazole preparation was evaluated for efficacy against strongyles in 107 adult horses under stable conditions by egg counts before and after treatment. Seven treatment courses were used: 20g×2×1 (20g of the preparation per head b.i.d. for one day), 20g×1×2, 10g×2×1, l0g×1×2, 5g×2×1, 5g×1×2, and 1g×1×10. Each horse was subjected to 4 treatments of one of 7 courses at intervals of about 3 months during about one-year period, irrespective of the level of egg count. No distinct differences were observed in efficacies among the 7 treatment courses. Egg counts obtained intermittently for about one year after the last treatment revealed that 4 treatments during a one-year period might exhibit continued suppressive effects on the strongyle populations until about 8 months after the last treatment. Strongyles excreted from 2 horses treated with 10g×2×1 included 15 species which have been ordinarily encountered in Japan. From a practical viewpoint 10g×2×1 or 10g×1×2 may be a treatment course of choice.
Four piglets 5 months old were inoculated subcutaneously with 106.0 TCID50/ml of the HT--SK-C strain of porcine parvovirus (PPV) that had been adapted to primary swine kidney cell culture at low temperature. None of them presented any abnormal clinical sighn, viremia or virus discharge. Four weeks after inoculation, 2 of them and a cohabiting control piglet were challenged orally with 106.0TCID50/ml of a field strain (90HS strain). One of the inoculated piglets exhibited weak viremia, but not leukopenia or virus discharge. The control piglet exhibited viremia, leukopenia and virus discharge. HI and neutralizing antibody titers rose rapidly in it after challenge. HI antibody titer increased to 1: 160-1: 640 and neutralizing antibody titer to 1: 64-1: 256 2-3 weeks after inoculation. HI and neutralizing antibody titer were 1: 80 and 1: 16-1: 64, respectively, 12 weeks after inoculation.
Four swine 7 months old died suddenly. Autopsy on one of them revealed bubbles on the liver and sponge-like appearance of the cut surface of this organ. Bacteriologically, 104-108 organisms of Clostridium novyi type A were isolated from all the organs examined. To make a rapid diagnosis, gas chromatography was carried out to find propionic acid as a major peak, acetic acid and butyric acid. When labelled antiserum to C. novyi was used, specific fluorescence was detected from frozen main organs and blood.
A tissue specimen was allowed to contact the freezing stand of the cryostat directly when the cryostat was held at 25°C. Thin frozen sections were prepared from the outermost part of the frozen surface of the specimen. They were rather free from artifacts. Satisfactory sections were obtained from an outermost part less than 0.5 mm deep from the frozen surface of a muscle specimen or less than 2.0mm deep from that of a specimen from the liver or kidney.