In March 1995, 1.27% of broilers examined from a flock showed bleeding in the legs, and anavian reovirus was isolated from tendon with tenosynovitis. The isolate killed all specific-pathogen-free (SPF) chicken embryos after inoculation into the yolk sac with 10-104plaque forming units (PFU), and 7 of 8 8-day-old SPF chicks showed tenosynovitis after inoculation with 106 PFU into the pad. Sera from this flock showed neutralizing antibody titers of 1: 274, and 1: 2, 694 to TS-142 and the isolate, respectively. Six of 17 near by broiler flocks as well as all 38 layer flocks examined, were infected showing serum neutralizing antibody titers of 1: 20 to 1: 1, 280≤.
In a dairy farm in which 169 cows were raised, bovine mastitis due to Mycoplasma bovis occurred. Mycoplasmas were isolated from 5 milk samples out of from which no bacteria were isolated.In the neighboring farm, no mycoplasma was isolated from cows with clinical mastitis resisting to antibiotic treatments, but antibodies to M. bovis were detectable by indirect hemagglutination test in 30 of 100 (30%) sera.
A metabolic profile test after parturition was performed on Japanese black dams of 7 farms, from days 8 to 99 (stage I), from days 100 to 199 (stage II) and, from days 200 or later (stage III). In group A (41 dams of 3 farms) given corn silage throughout the year, the calves experienced frequent occurrence of white scour and the dams showed abnormalities in rumen conditions and decreased fat metabolism through the stages I to III. In group B (22 specimens of 2 farms) given corn silage except the stage I after parturition many cases ofthe disease were observed in calves, and energy balance remained lower at the stage I and fat metabolism deteriorated over the stages I to II in dams. The results suggested that decreased fat metabolism in dams during the stage I was relative to the occurrence of white scour in calves.
Broiler cases of swollen head syndrome (SHS) occurring at three different farms and showing respiratory symptom, swollen heads and severe depression were studied pathologically. Atnecropsy affected chickens showed the swollen head and eyelids due to facial cellulitis with caseous exudate in the air space of the cranial bones but no exudate in the lumen of the infraorbital sinus and the nasal cavity. Microscopy revealed chronic fibrinopurulent inflammation with granulomas in the air space of the cranial bones, middle ears and facial skin. Conjunctivitis and tracheitis with infiltration of lymphocytes and plasma cells with germinal centers were observed. The E. coli isolate from the wattle of an affected chicken was inoculated intramuscularly (107 CFU) into 2-weeks-old SPF chicks, and acutefibrinopurulent pericarditis and serositis, but no SHS lesions were produced.
Cows were given the control diet (Group A, 7 cows) high protein, and diet (Group CP, 5 cows) or high starch diet (Group ST, 6 cows), and blood was sampled and examined by chemical components before, 2 hr after, and 4 hr after the morning feeding. In Group A blood sampled 2 hr after feeding showed lower contents of total cholesterol (TCHO), beta lipoprotein (β-LIPO), and ammonium (NH3) as compared with samples before feeding. In Group CP samples at 2 hr postfeeding were higher in blood urea nitrogen (BUN) and lower in glucose (GL), while higher contents of triglyseride (TG) and BUN were seen in Group ST. In Group contents of CP, TG, β-LIPO and NH3 were higher than those in Group A at 2 hr postfeeding. At 4 hr after feeding such temporary fluctuations were much smallerin range, indicating that blood should be sampled for metabolic profile test (MPT) at 4 hror later after the morning feeding but before the next feeding.
By tubal-insufflation technique to see the oviduct patency and subsequent fertility in 56 Holstein repeat breeder cows, 5 types of the CO2 kymograph curves were obtained. Of 22 (39.3%) cases with the patent oviducts and 4 (7.1%) cases having temporary impassibleness with mucus and/or secretion, 14 and 2 cases became pregnant after the test, respectively. Eighteen (32.1%) cases with bilateral impassibleness of oviducts and 7 (12.5%) cases with unilateral impassibleness were slaughtered. The test was unsuccessful in 5 (9.0%) cases due to the gas escape thorough the cervix.
An eight-year-old female mongrel dog with symmetric diffuse hyperplasia around the upper jaw molar gingiva, was treated by removing dental plaque and dental calculus as well as root planing. Also gingivectomy and gingivoplasty were done. Histopathology of the resected sample revealed gingival hyperplasia. Thirteen months later, slight gingival hyperplasia appeared in proportion to dental deposition, and 3 years after the operation, the gingiva showed moderate hyperplasia in proportion to gradually increased dental deposition and severer hyperplasia in the region with a lot of dental deposition. No dental home care was made
In slaughtered meat pigs, the number and maturity of blood leukocytes and the neutrophilicalkaline phosphatase (NAP) activity were examined as compared with pathologic lesions, forestimating the cleanness of the growing and fattening environment. While the leukocyte count and neutrophil maturity were not correlated to detected pathologic lesions, the NAP activity reflected detectable lesions, suggesting that it might be useful for estimating the cleanness of swine farms.