Examination was made of variation in serum lipid peroxide, α-tocopherol level and superoxide dismutase activity in dairy cattle, fattening cattle and cattle with liver disorders. In the fattening group, serum lipid peroxide levels markedly increased before and after parturition and exceeded 15 nmol/ml at 12 weeks after parturition. In the control group, no significant variation in serum lipid peroxide levels in association with parturition. Serum α-tocopherol levels and superoxide dismutase activity in the fattening group changed remarkably, compared to control. These parameters trasiently or persistanly decreased before and after parturition. Serum lipid peroxide levels in Japanese black cattle at the later stage of breeding were measured for two cattle groups reared under different breeding conditions. Although there were no difference in serum superoxide dismutase activity in the 2 groups, serum lipid peroxide levels increased in the group whose serum α-tocopherol levels decreased. Serum superoxide dismutase activity in cattle with liver damage remained higher throughout the period of observation, than the control, being as much as 35% higher at the onset of liver disorder. Serum α-tocopherol decreased to less than 100 μg/dl after liver damage had been sustained and at 10 months before the onset when serum lipid peroxide increased.
There occurred an incident of respiratory disease at a cattle raising station in Yamaguchi Prefecture during the period from September to October, of 1988. Seventy-seven dairy calves, 60 to 150 days of age, developed respiratory symptoms acconpanied by fever, coughing and nasal discharge. Four cytopathogenic virus strains were isolated in Vero cell cultures from nasal secretions of 12 affected calves and identified as bovine respiratory syncitial virus (BRSV) based on physiochemical properties and results obtained by the fluorescent antibody technique and neutralization tests using a specific immune serum against BRSV. In six of 12 paired serum samples, the neutralizing antibody titer against BRSV increased significantly. The respiratory disease had proceeded unchecked for about a month following the first outbreak. The average duration of the symptoms was approximately 10 days.
Clinical and pathological examinations were conducted on a 21-month-old fattening ox with endocardial fibroelastosis. Auscultationally, the first and second sounds were of sufficient intensity to be heard and there was also a loud systolic murmur. An Elecrocardiograph indicated prolonged P waves and a QRS complex and increased amplitude of T waves. A phonocardiograph gave indication of crescendo-decrescendo systolic murmur in the vicinity of the bilateral atrio-ventricular valves. Marked hypertrophy of the heart was evident by ultrasonography. The results of autopsy showed marked hypertrophy of the heart and whitish and diffuse lesions of fibrotic thickening on the endocardium of the right atrium, ventricle, and chordae tendineae. Focal fibrotic lesions were also present on the endocardium of the left ventricular outflow tract. Microscopical examination demonstrated proliferation of collagen and elastic fibers in the endocardium of the bilateral atrium, ventricle, and sub-endocardial interstitial tissue. Thickening of arterial walls was also evident.
Arteriosclerosis was noted in 18, 3-to 6-month-old, fattening pigs and 13, 3-to 8-year-old, breeding pigs. In all cases, autopsy indicated rough intimal surface of the abdominal aorta and its bifarcation. The arteriosclerotic lesions were histologically characterized by cellular and fibrous thickening of the aortic intima which was comprised mainly of proliferated smooth muscle cells and fibrous elements. Intimal thickening incleased with age of the animals but no athero-sclerotic lesions could be found in any of the pigs. Hyaline deposition was found in the intimal thickening of young fattening pigs and aging breeding pigs. Histochemical analysis of the hyaline substance indicated a protein containing neutral mucopolysaccharide to be present, indicating a possible metabolic disorder of glycoprotein in intimal smooth muscle cells. The vascular lesions in the animals appeared due to high-protein and high-energy food provided to the pigs in excessive amount.
Renal biopsy, conducted on two cats that presented critical proteinuria, hypoproteinemia, edema, and thrombocytopenia, disclosed mesangioproliferative glomerulonephritis in both cases. One showed deposition of immune complex while the other deposition of anti-glomerular basement membrane antibody. Treatment with prednisolone markedly improved urinary and serum protein levels, dissolving edema in each case. However, one case developed a serious thrombocytopenia and died from bleeding.