1. It may be said that no feed should be given, “in such a small amount as to cause the presence of ketones in the urine or in such a large amount as to incur the presence of indican in the urine.” It was impossible to determine accurately the limit of feeding from the results obtained from this survey. However, so far as the limit of feeding is concerned, it is considered with a common-sense view that both DCP and TDN may be in the neighborhood of 1.1 to 1.2 according to MORIMOTO's provisional method for the calculatictn of NRC feed. In addition, enlightenment and extension work is needed for the correction of a habitual way of feeding high-protein ration by the maintenance of supply of carbohydrates of superior quality.
2. It should be recommended to give silage and other fermented feed as source of supply of roughage during the winter. The veterinarian and the animal husbandman are expected to study in more detail the method of preparation and storage of such feed and to extend adequate guidance to this effect to the farmer.

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Isolation of halophilic bacteria was conducted on 85 samples of marine products. As a result, 43 strains of Gram-negative bacilli were isolated on mannite-salt agar (salt concentration: 7.5 per cent). Of these strains, 42 grew on ordinary agar media, but one (No.33) did not; 31 strains fermented mannitol.
Strain No.33 was of Gram-negative bacilli, which grew on mannite-salt agar but not on ordinary agar media. This strain was examined for biological characters. The results indicated that the organism of this strain was a motile, pleomorphic rod producing aerobic growth on ordinary agar media. It was hemolytic, produced no pigments soluble in culture media and no indole, and caused no coagulation of milk media and no reduction of potassium nitrate. It grew well in peptone water containing 7.5 per cent salt and decomposed glucose.
Judging from the biological characters mentioned above, the isolated strain No.33 is considered to belong to the pseudomonas group. It is also similar the Takikawa strain which was described by Takikawa as a causative agent of food poisoning.
In short, only one strain of halophilic: bacteria was isolated from the 85 samples of marine products investigated.

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It is said that the bacteria contaminating semen cause a lower rate of conception than usual. Recently, Straw's method has come into use to effect the deep injection of semen. Especially in this case, bacterial contamination is worthy of attention.
The authors conducted a field survey on the state of contamination of semen and investigated the effect of antibiotics on the collection, treatment, and storage of semen in order to find any preventive measure against contamination
1) The results of the survey indicate that the semen can be protected from bacterial contamination to a large extent when the semen-collecting enclosure and the stand to be used at the time of collection are equipped with sanitary facilities and the hind quarters of a teaser cow are cleaned, when animals are prevented from irritation and uproar, and when the inner side of the prepuce of the bull is thoroughly washed.
2) The smaller the extent of bacterial contamination, the more remarkable was the effect of SP drug. When contamination was extreme, little effect was observed of SP drug.
3) The effect of SP drug was observed more conspicuously when the semen diluted with the diluent containing SP drug was stored for 4 to 6 days than immediately after the semen was diluted with the same diluent containing SP drug.

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