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Volume 53, Issue 9
Displaying 1-9 of 9 articles from this issue
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2000 Volume 53 Issue 9 Pages 567-575
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (5800K) -
Fuminori NAGAI, Hiroyoshi OTA, Katsuhisa FUJIMOTO, Hideo ABE, Takumi M ...2000 Volume 53 Issue 9 Pages 577-581
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSVerrucous dermatitis and digital papillomatosis caused by Treponema-like spirochetes occurred in 43 dairy cattle on a farm in the Ishikari district, Hokkaido. Located mainly on the hind limb, the almost circular, raised lesions encrusted the plantar aspect of the pastern. Inflicted animals frequently demonstrated signs of lameness in the hind limb. The lesions were malodorous and very painful when touched. In the keratinized outer layer of the epidermis, histopathological examination revealed hyperkeratosis and parakeratosis with multiple foci of bacterial infection associated with perivascular infiltration of mononuclear cells in the dermis. Levaditi silver impregnation and Warthin-Starry staining of histological sections detected organisms with spirochete morphology in the shallower region of the epidermis. In terms of immunohistochemistry, the organisms reacted positively to the anti-Treponema pallidum polyclonal antibody. The ultrastructural morphology of the spirochetes resembled that of the Treponema sp. Though they responded strongly to antibiotic treatment with tetracycline, cefazolin, and chloramphenicol, in many cases, the lesions recurred in from 1 to 3 months after therapy. We failed to isolate the pathogen from the lesions.View full abstractDownload PDF (3344K) -
Kyoji YAMADA, Toshihiko NAKAO, Gen MATUSDA, Toshinori TANAKA, Touki TO ...2000 Volume 53 Issue 9 Pages 582-585
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe aim of this study is to assess reproductive performance in dairy cows after OVSYNCH/Timed Artificial Insemination (OVSYNCH/TAI) protocol and to determine whether numbers of post-calving days, parity, and season affect it. Subjects were 556 Holstein dairy cows from 24 herds. The cows had either not been observed to be estrous after calving or had not conceived upon artificial insemination (AI) before being subjected to OVSYNCH/TAI. Of the 556 cows, 41 (7.4%) were in heat between the initial GnRH injection and PGF2α, 7 days later. Most of them were observed to be in estrus 6-7 days after GnRH. Of these 41 cows, 16 were artificially inseminated at this heat; and 5 (31.3%) conceived. A total of 513 cows was inseminated artificially at fixed times after OVSYNCH. The conception rate for this timed-AI was 55.4%. No effects caused by numbers of post-calving days, parity, and season were observed. In conclusion, du ing the OVSYNCH protocol, about 7% of the animals subjected to the procedure can be observed to be in estrus; and numbers of post-calving days, parity, and season exert no effect on conception rates after OVSYNCH/TAI.View full abstractDownload PDF (662K) -
Noboru ITOH, Masateru KOIWA, Rikio KIRISAWA, Hiromichi OHTSUKA, Takato ...2000 Volume 53 Issue 9 Pages 586-589
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA case of infection with the bovine viral diarrhea virus 1 (BVDV1) with clinical signs of infection with the BVD virus 2 (BVDV2) was observed on a farm on Hokkaido. The affected cow showed thrombocytopenia, fever, and petechiae in the mucous membrane, diarrhea, and leukopenia after calving. The neutralizing antibody titer to the BVDV1 was 2, 048, but no virus was isolated from BFM (bovine fetal muscle) cell cultures inoculated with the animal's serum. Sequencing of PCR products revealed the virus to be BVDV1. These findings suggest that BVDV1 infection and/or calving may cause clinical symptoms.View full abstractDownload PDF (1414K) -
Yukinori UCHINUNO, Kazuki ISHIBASHI, Yasuharu HIRAKI, Keiichi GOTO, Ei ...2000 Volume 53 Issue 9 Pages 590-592
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIbaraki virus (IBAV) prevailed on certain dairy farms between June 1997 and March 1998. During that time, surveys were conducted among un-inoculated dairy cows (24-28) and inoculated dairy cows (24-27) to determine the presence of neutralizing antibodies to the epizootic strain 97MD70 and non-epizootic strain No.2. In September and October, positive antibody rates for both strains were high. Neutralizing antibody titers to both the 97MD70 and No.2 strains were significantly lower in inoculated cows than un-inoculated cows (P<0.01). Epidemiological investigations conducted on serum samples collected from cattle spending the first summer seasons of 1997 and 1998 in the region showed neutralizing antibody titers to the No.2 strain to be significantly lower than titers to the 97MD70 strain (P<0.01). Among examples surveyed periodically, a maximum of 40 out of 99 (40.4 %) samples obtained in September 1997 was antibody-positive to the 97MD70 strain but negative to the No.2 strain. These findings suggest that the epizootic strain should be used in neutralization test with IBAV for serological survey.View full abstractDownload PDF (481K) -
Takafumi ONISHI, Hisashi INOKUMA, Koichi OHNO, Shingo SOEDA, Kazuhiro ...2000 Volume 53 Issue 9 Pages 595-601
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSPlasma C-reactive protein (CRP) concentrations were measured in 207 normal and 442 diseased dogs by means of laser nephelometric immunoassay developed for private use in canines and already commercially available. The measurements determined the canine plasma CRP cut-off value as 1.0 mg/dl. So far, no differences related to breed, age, or sex have been noted. Abnormally high CRP values were observed in dogs with various acute generalized infectious diseases, immune-mediated or allergic diseases, tumors, endocrine or metabolic diseases, uremia, and other inflammatory diseases or traumas. Normal or low CRP values, however, were observed in various non-inflammatory diseases or focal or chronic inflammatory diseases. Measurement of CRP by serial repetition was found to be an essential tool in assessing therapeutic effects and in prognosticating. In addition, it can be useful as a better marker of inflammation than white-blood-cell counts.View full abstractDownload PDF (1128K) -
Yoshiaki NAGAYA, Takashi HASHIMURA, Mio KAKUI, Naomi INOMATA, Seiji TA ...2000 Volume 53 Issue 9 Pages 602-604
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSOn the basis of various clinical tests, including computerized tomography (CT) scanning, a nine-year-old male Maltese dog suffering from seizures was diagnosed as having a brain tumor. Craniotomy led to tumor resection and decreased intracranial pressure. Histopathological examination resulted in a diagnosis of malignant astrocytoma.View full abstractDownload PDF (2205K) -
Makio MIZOBE, Yoshifumi SENOKUCHI, Kazuhiko IKI, Tadayoshi TOKUI, Naom ...2000 Volume 53 Issue 9 Pages 607-613
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1125K) -
[in Japanese]2000 Volume 53 Issue 9 Pages 619
Published: September 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (874K)
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